introduction goals & myself
introduction, goals & myself
Andreas Manz
h l f f b
FRIAS School of Soft Matter, Freiburg Germany
KIST Europe, Saarbrücken Germany
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part 1
part 1
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miniaturization
down-scaling
engineering
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No content
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objects which have a reason for
their si e
their size
?
?
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objects which don’t have a reason
for their si e
for their size
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typical lengths
yp g
5
1
1/20
1/20
1/200
1/200,000
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human perception
p p
< 1 cm is small
< 1 cm is small
> 2 m is large
g
< 100ms is fast
> 10 i i l
> 10 min is slow
“l b hi ”
“lab on a chip”
microfabrication
microfabrication
microfluidics
μTAS
miniaturized total analysis systems
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capillary electrophoresis on chip
1989-91
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flow injection analysis, 1989-92
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for more information on the topic
for more information on the topic
micro TAS conference
G i th
Groningen, the
Netherlands 2010
impact factor 6.5
1,000 attendees annually
p f
Reviews on Micro total analysis systems
in Anal.Chem. 2002, 04, 06, 08 and 10
cited over 3,000 times
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part 2
part 2
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electrophoresis
p
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10 fold miniaturization
100 x faster separation
p
1000 x smaller volume
10 x lower reagent consumption
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1747
1747
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double stranded DNA separation
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reaction with intercalating dye
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part 3
part 3
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„the human document project“
p j
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„the human document project“
p j
goal
t d 1 d t b t h
•to produce 1 document about human
culture, science, arts etc.
culture, science, arts etc.
•to preserve it for 1 million years
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keep an eye on
p y
time scale !
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1 million years back
1 million years back
Homo neanderthalensis
Homo sapiens
Homo heidelbergensis
Homo habilis
Homo erectus
Paranthropus
Ardipithecus
Australopithecus
6 5 4 3 2 1 0 million years
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1 million years back
1 million years back
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venus statuettes, cave paintings
pottery
pottery
stone axes
pebble tools
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„the human document project“
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Lespugue, upper paleolithic, 25,000 B.C.
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„the human document project“
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Lascaux cave, upper paleolithic, 14,000 B.C.
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„the human document project“
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Tartaria tablets, 5,500 B.C. Jiahu symbols, 6,000 B.C.
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„the human document project“
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Epic of Gilgamesh
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begins with instructions on
how to find a box of copper
ow to d a bo o coppe
inside a foundation stone in the
great walls of Uruk
3,000 B.C.
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„the human document project“
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Egyptian tombs are also time capsules
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„the human document project“
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time capsule
1939
crypt of
civilization,
Oglethorpe
University,
Atlanta Georgia
Atlanta, Georgia
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„the human document project“
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Pioneer plaque 1972, Voyager CD 1977
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„the human document project“
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Th L N F d ti D i l
The Long Now Foundation Daniel
Hillis, San Francisco
,
The Ozymandias Project David Green,
New York
New York
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…books last a few hundred years
…boo s s ew u d ed ye s
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…or a few thousand
…o ew ous d
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…ceramics or stone last longer
…ce cs o s o e s o ge
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old monocrystalline material
y
lasted for millions of years
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deep etching of monocrystalline silicon
deep e c g o o oc ys e s co
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embedding it in amber
e bedd g be
resin source today
Agathis australis (New Zealand)
monomer: communic acid
monomer: communic acid
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the oldest digital document on this planet
e o des d g docu e o s p e
… is DNA
d t i
and proteins
for many protein sequences,
the evolution can be tracked
the evolution can be tracked
back 1,000,000,000 or
2,000,000,000 years
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age of proteins
ge o p o e s
Evolutionary Horizons (Million years)
Fibrinopeptides 200 Mammalian Radiation
L t lb i 670 V t b t
Lactalbumins 670 Vertebrates
Ribonucleases 850 Animals
Hemoglobins 1 500 Plants/Animals
Hemoglobins 1,500 Plants/Animals
Acid Proteases 2,300 Prokayrotic/Eukarotic
adapted from R.F.Doolittle et al., Cold Spring Harb. Symp. Quant. Biol.,
51:447–455, 1986
,
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age of proteins
ge o p o e s
source W. R. Pearson (2000) "Protein sequence comparison and protein
evolution“ ISMB2000 - Tutorial, San Diego, CA.
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encoding it in DNA
e cod g N
deep sea shark
deep sea shark
long generations
little variation in DNA
link to essential protein
link to essential protein
or: abundant microorgansim?
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dumping Wikipedia
dumping Wikipedia
on the moon
meteorite-safe deposit
meteorite safe deposit
labelling location visible from earth
lti l i
multiple copies
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what kind of information will be
important to mankind in 1 million years?
important to mankind in 1 million years?
subjective or objective?
what defines / describes our culture of
h d t d ?
here and today?
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how to make sure, it can be understood?
Rosetta stone-like or children’s learning-
like?
like?
computer code, English or pictograms /
h t ?
photos?
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learning / definitions
Lik R tt t
Like Rosetta stone
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learning / definitions
lik hild ’ l i
like children’s learning
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where to distribute it? in what numbers?
how to get attention?
how to get attention?
it shouldn’t be found too early, or too
late / not at all?
late / not at all?
near Gizeh? London? Baltic Sea? Gobi
d t b f Chi ?
desert or urban areas of China?
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attract attention
c e o
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attract attention by fluorescence
( ti l / t d t )
(nanoparticles / quantum dots)
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include a timer
cocktail of radioactive isotopes?
p
description of our calender and
astronomic event?
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include a timer
cocktail of radioactive isotopes?
aluminium26, magnesium26 720.000y
uranium234, thorium230 80.000y
, y
uranium235, protactinium231 34.300y
carbon14 5.730y
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one astronomic event…
Easter
Island
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… may not be enough
Easter
Island
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should it be a monograph?
could it be parts of a puzzle, referring to
each other?
each other?
d fi i i i d i / l i d
definitions, introduction / learning and
then chapter in maximum encoding
e c p e u e cod g
density?
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brainstorming
brainstorming
workshop
June 30- July 2, 2010
Saarbrücken
Saarbrücken
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purpose of this workshop
what do we want?
what do we want?
who are we?
di th bl
discuss the problems
networking
g
“white paper” - goals
“road map” time frame milestones
road map – time frame, milestones
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purpose of this workshop
“white paper”
white paper
define goals
ll
overall
for each discipline
p
(create a paper for ourselves, funding
bodies press students)
bodies, press, students)
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purpose of this workshop
“road map”
d fi k
define tasks
disciplines, regions, indivduals
p , g ,
avoid too much duplication
ifi ll b ti
specific collaborations
kick start the “bigger”
gg
(put together what we intend to do)
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publications
open access e-only journal
“Ch ll ”
“Challenges”
published by MDPI Basel
p y
special issue “Human Document”