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DNA (DBCLS) 2018/12/26 JST

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ࠓ೔ͷ͓࿩ ੜ෺ֶNFBUT৘ใՊֶ

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http://docman.dbcls.jp/im/

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ٕज़ͷਐา͕ή ϊϜՊֶͷઓུΛม͑ͨ ೥͝Ζ

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Ή͔͠αϯΨʔ๏ʹΑΔ%/"഑ྻղಡ Ͱ͖Δ͜ͱ͔Βͭͭ͜͜ͱ

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͜ΕΛઃܭ͠ͳ͍ͱಡΊͳ͍ ग़య: https://www.researchgate.net/figure/Principle‐of‐Sanger‐sequencing‐Incorporation‐of‐ ddNTPs‐terminates‐the‐elongation‐of‐the_fig4_265264877

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͍·େن໛Ԙج഑ྻղಡٕज़ʹΑΔ໢ཏతܭଌ ͱΓ͋͑ͣશ෦ಡΉ

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Ϳͭ੾Γʹ͢Δ શ෦ʹΞμϓλʔ഑ྻΛ͚ͭΔ શͯͷ഑ྻʹରͯ͠ ૬ิ࠯ͷ߹੒Λ͢Δ ೋ࣍ݩ্ۭؒͷܬޫΛ ΧϝϥͰࡱӨɺܦ࣌ͷ มԽΛॲཧͯ͠഑ྻʹ ม׵͢Δ ग़య: https://bitesizebio.com/13546/sequencing‐by‐synthesis‐explaining‐the‐illumina‐sequencing‐technology/

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ಡΊͳ͔ͬͨ΋ͷ͕ಡΊΔΑ͏ʹ ͱΓ͋͑ͣಡΜͰΈΑʂ

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ग़య: https://gold.jgi.doe.gov/statistics

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͋ͷੜ෺ͷή ϊϜͬͯ΋͏ಡ·Ε͚ͯͨͬʁ ή ϊϜห౰ϓϩδΣΫτ

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ग़య: https://www.instagram.com/p/BcybbydAbSK/

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ग़య: http://wiki.lifesciencedb.jp/mw/GenomeBentoProject

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େྔฒྻΛ࢖༷ͬͯʑͳݱ৅Λܭଌ͢Δ 4FRͷ࣌୅

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ϥϘ͔Βσʔλ͕ͲΜͲΜग़ͯ͘Δ ͕ͩγʔέϯα͸ੜମ෼ࢠΛจࣈʹม׵͢ΔػցͰ͋Γ஌ࣝΛੜΉ΋ͷͰ͸ͳ͍

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ੜ෺ֶͷ஌ࣝ ৘ใՊֶͷٕज़Ͱ จࣈྻͷฒͼΛ஌ࣝʹม׵͢Δ

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#JH%BUB4DJFODFͱͳͬͨੜ෺ֶ͸ܭࢉػͳ͠Ͱ͸΍͍͚ͬͯͳ͍ ੜ෺ֶNFBUT৘ใՊֶ

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Պֶతख๏ͷͭͷύϥμΠϜ ܦݧཧ࿦ܭࢉσʔλ

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Ծઆ͔Β࣮ݧΛσβΠϯ͢ΔPS·ͣσʔλΛऔͬͯ෼ੳ͢Δ Ծઆۦಈܕσʔλۦಈܕ

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ϝλή ϊϜ Կ͕ೖͬͯΔ͔Θ͔Μͳ͍͚Ͳ%/"શ෦ಡΊ͹Α͘Ͷʁ

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ւਫதͷ%/"͔ΒڕͷछྨΛਪఆ ஑ͷਫશ෦ಡΉ ग़య: https://doi.org/10.1098/rsos.150088-9

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ۭؾதͷ%/"͔Βඍੜ෺Λਪఆ ۭؾಡΉ ग़య: https://doi.org/10.1371/journal.pone.0001862

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ެڞۭؒͷ%/"͔Βඍੜ෺Λਪఆ .FUB46#1SPKFDU ग़య: https://doi.org/10.1186/s40168‐016‐0168‐z

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ഉᔔ෺தͷ%/"͔Β௎಺ࡉەΛਪఆ ʜ͕Α͘ߦΘΕΔ͕Լͷਤ͸໺ੜಈ෺Λই͚ͭͣʹ%/"Λ࠾औ͢Δख๏ͷ࿦จ ग़య: https://doi.org/10.1038/s41598-018-20427-9

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೥ౙɾ౎಺๭ॴ ݚڀूձʮୈ࢛ճ/(4ݱ৔ͷձʯاըձٞ

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ʮ͜Ε͚ͩγʔέϯε҆͘ͳͬͨΜ͔ͩΒͳΜ͔ಡ·ͳ͍ʁʯ

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ࡩͷ඼छͰ͋ΔιϝΠϤγϊ͸઀͗໦Ͱ૿͑ΔΫϩʔϯ Ұఆͷؾ৅৚݅ͰҰ੪։Ֆ͢Δ͕Ұि͔ؒΒे೔Ͱࢄͬͯ͠·͏

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॓ओͷή ϊϜ͸ಉ͡Ͱ؀ڥ৚͚͕݅ͩҟͳΔ ෇ணͨ͠%/"ͷҧ͍͸؀ڥͷҧ͍Λ൓ө͢Δ͔ʁ

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ͱ͸͍͑ҋӢʹγʔέϯε͢Ε͹Α͍ͱ͍͏Θ͚Ͱ͸ͳ͍ͷͰ ݚڀσβΠϯ Λ͠·͢

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༧ࢉ૊Έ αϯϓϦϯάΩοτͱࢼༀͷௐୡ αϯϓϦϯάΛ͢Δ αϯϓϧ͔Β%/"Λநग़͢Δ 4S3/"ྖҬΛ૿෯ͯ͠γʔέϯε͢Δ ੜ෺෼ྨσʔλϕʔεʹ໰͍߹ΘͤͯಡΜͩ഑ྻ͔Βੜ෺छΛਪఆ͢Δ ֶձͰղੳίϯϖςΟγϣϯΛ΍Δ ࿦จΛॻ͘

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Ϋϥ΢υιʔγϯά શࠃ͔ΒࢀՃऀΛืͬͯαϯϓϦϯάΛ͓ئ͍͢Δ

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༧ࢉ૊Έ αϯϓϦϯάΩοτͱࢼༀͷௐୡ ΢ΣϒΞϓϦέʔγϣϯͷ࣮૷ͱެ։ એ఻ͱࢀՃऀืू ΩοτΛࢀՃऀʹૹΔ αϯϓϦϯάͯ͠΋Β͏ αϯϓϧΛૹͬͯ΋Β͏ αϯϓϧ৘ใΛॻ͍ͯొ࿥ͯ͠΋Β͏ ૹΒΕ͖ͯͨαϯϓϧ͔Β%/"Λநग़͢Δ 4S3/"ྖҬΛ૿෯ͯ͠γʔέϯε͢Δ ੜ෺෼ྨσʔλϕʔεʹ໰͍߹ΘͤͯಡΜͩ഑ྻ͔Βੜ෺छΛਪఆ͢Δ ֶձͰղੳίϯϖςΟγϣϯΛ΍Δ ࿦จΛॻ͘

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αϯϓϦϯάࢀՃऀ 149 ໊

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ू·ͬͨαϯϓϧ਺ 594

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શࠃ੍೼ (ԭೄΛআ͘)

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ಡΜͰΈͨ

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ಘΒΕͨ഑ྻͷ΄ͱΜͲ͕ ༿྘ମͱϛτίϯυϦΞ ίϯλϛͩ

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ίϯλϛ $POUBNJOBUJPOԚછ Պֶ࣮ݧҰൠʹ͓͍ͯҙਤ͠ͳ͍෺࣭͕ࠞೖ͢Δ͜ͱ

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ɾТɾʆ  Ұൃউෛͩͱ͜͏͍͏͜ͱ΋͋Δ

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αϯϓϦϯά࣌ʹՖͼΒͷද໘Λ ࡲΓ͗ͯ͢ࡩͷࡉ๔͕ࠞೖͨ͠ͱ ࢥΘΕΔͷਤ

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࡞ઓมߋ ݟ͔ͭͬͨ഑ྻͷதʹԿ͔໘ന͍΋ͷ͸ͳ͍͔ʁ

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΄ͱΜͲ༿྘ମͱϛτίϯυϦΞ·ΈΕ ΄΅ίϯλϛͱ૝૾͞ΕΔ͕ɺશ෦͕ͦ͏ͳͷ͔ʁ

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ҧ͏২෺ʹ༝དྷ͢ΔͱࢥΘΕΔ഑ྻ γʔέϯεͷϛεͷՄೳੑ΋͋Δ͕ɺϛεϚονͷ਺Λߟ͑Δͱʜʁ

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Cryptomeria japonica Ֆ࣌ӍࡩࢄΓΏͦ͘ͷલʹ

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ɾТɾʆ  ͜͏ͯ͜͠ͷੈքʹ·ͨҰͭ৽ͨͳτϦϏΞ͕ੜ·Εͨʜ

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ر๬͸ͳ͍ͷ͔ ͜ͷ··Ͱ͸কདྷʹͭͳ͕Βͳ͍

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ܚጯٛक़େֶઌ୺ੜ໋Պֶݚڀॴ ߥ઒άϧʔϓ ߥ઒ Տ໺ ౻ݪ ٢ా ߴҪ Ѩ෦ ࠤ౻ণ௚͞Μ

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ٕज़һ ٕज़һ ֶੜ ॿڭ ॿڭ ॿڭ ।ڭत ൿॻ 0 50 100 150 200 count abe Arakawa Fujiwara Ishii Kono sato Takai Yoshida member * * εʔύʔςΫχγϟϯҰਓউͪ ݸਓຖͷαϯϓϦϯάʹ͓͚ΔόΫςϦΞ഑ྻ਺ ߥ઒࿨੖͞Μ ܚጯٛक़େ

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0 1000 2000 3000 count abe Arakawa Fujiwara Ishii Kono sato Takai Yoshida member 0 50 100 150 200 count abe Arakawa Fujiwara Ishii Kono sato Takai Yoshida member ٕज़һ ٕज़һ ֶ ॿڭ ॿ ॿڭ ।ڭत ൿॻ ٕज़һ ٕज़һ ֶ ॿڭ ॿ ॿڭ ।ڭत ൿॻ ༿྘ମɾϛτίϯυϦΞআ֎ ༿྘ମɾϛτίϯυϦΞؚΉ ՖคΛଟ͘ͱͬͯ͠·͍ͬͯΔ όΫςϦΞΛ༏ઌతʹͱΕ͍ͯΔਓ͕͍Δ 10 15 20 25 30 35 40 45 50 55 4 500 1000 1500 2000 2500 17 20 40 60 80 100 4 0 500 1000 1500 2000 2500 3000 17 0 10 20 30 40 50 4 0 250 500 750 1000 1250 1500 17 0 10 20 30 40 50 4 0 250 500 750 1000 1250 1500 17 0 5 10 15 20 25 30 4 200 400 600 800 1000 1200 1400 17 0 5 10 15 20 4 0 200 400 600 800 1000 1200 17 0 50 100 150 200 4 1500 1750 2000 2250 2500 2750 3000 17 0 20 40 60 80 100 120 4 0 500 1000 1500 2000 17 ٕज़һ ٕज़һ ֶੜ ॿڭ ॿڭ ॿڭ ।ڭत ൿॻ ༿྘ମɾϛτίϯυϦΞͷ഑ྻ਺ͱόΫςϦΞͷ഑ྻ਺ͷ૬ؔ ߥ઒࿨੖͞Μ ܚጯٛक़େ

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഑ྻσʔλɺϝλσʔλɺ࿦จͦͷଞશ෦͜͜ʹ͋Γ·͢ EBUBECDMTKQdJOVUBOPPNHTFR

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αϯϓϦϯάͷख๏Λ޻෉͢Ε͹ΑΓΑ͍σʔλ͕ಘΒΕΔ ͔΋  SF͓ՖݟϓϩδΣΫτ ٻΉϓϩδΣΫτϦʔμʔ

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Պֶݚڀͱͯ͠੒ཱͤ͞Δ͜ͱ͚ͩͰ͸ͳ͍ ͓ՖݟϓϩδΣΫτਅͷ໨త

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༧ࢉ૊Έ αϯϓϦϯάΩοτͱࢼༀͷௐୡ ΢ΣϒΞϓϦέʔγϣϯͷ࣮૷ͱެ։ એ఻ͱࢀՃऀืू ΩοτΛࢀՃऀʹૹΔ αϯϓϦϯάͯ͠΋Β͏ αϯϓϧΛૹͬͯ΋Β͏ αϯϓϧ৘ใΛॻ͍ͯొ࿥ͯ͠΋Β͏ ૹΒΕ͖ͯͨαϯϓϧ͔Β%/"Λநग़͢Δ 4S3/"ྖҬΛ૿෯ͯ͠γʔέϯε͢Δ ੜ෺෼ྨσʔλϕʔεʹ໰͍߹ΘͤͯಡΜͩ഑ྻ͔Βੜ෺छΛਪఆ͢Δ ֶձͰղੳίϯϖςΟγϣϯΛ΍Δ ࿦จΛॻ͘

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$

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೔࣌΢ΣϒΞϓϦέʔγϣϯ͕ࣗಈೖྗ αϯϓϦϯάͷ৔ॴ Ң౓ܦ౓ߴ౓ (14Ͱࣗಈೖྗ ఱީ೔࣌ͱ(14৘ใΛݩʹؾ৅ி%#͔Βநग़ͯࣗ͠ಈิ׬ ։Ֆޙͷ೔਺೔࣌ͱ(14৘ใ͔Βಉ͘͡ࡩ։Ֆ଎ใΑΓࣗಈิ׬ ͦͷଞͷ৘ใࣗ༝هड़

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ϓϩͷݚڀैࣄऀ͕ಉ͡ର৅ʹ͍ͭͯௐ΂Δ ؍࡯ͱهड़ͷ࣭ͱྔ

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ඈௗࢁଆ ࢪઃೖΓޱ ࢪઃෑ஍಺ JRԦࢠӺଆ ύʔΫαΠυ λϫʔαΠυ ιϝΠϤγϊ ಓ࿏Ԋ͍ೖΓޱ ߴ౓80.5m αΫϥͷՖ1ݸ෼ ιϝΠϤγϊݩ໦ $BTFT4JNQMFUFSNTPOMZ ղऍ͸೉͍͕͠ɺػց͕ಡΊΔ

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000353(ը૾000353_1)ʹ͍ͭͯɻ000361ͱͷ૬ҧ఺:ڳߴ௚ܘ56cmɻथߴ10.02m(ը૾000353_2தஈ)ɻथྸ͓Α ͼ২࠿ޙͷ೥਺͕ߴ͍ͱਪఆ͞Ε·͢ɻը૾ڳߴ෦ҎԼͷथൽ্ͷணੜ২෺౳ͷඃ෴཰80%௒ɻணੜ২෺౳͸ϋ ϦΨωΰέՊͳͲ᥌ଽྨ,ϊΩγϊϒͳͲ༽ࣃྨ,΢ϝϊΩΰέ΍ϨϓϥΰέͳͲ஍ҥମ͕ओͰɺΩϊίྨ͸ݟ౰ͨ Γ·ͤΜͰͨ͠(ը૾000353_3)ɻৗ྘޿༿थΛओͱ͢Δೋ࣍ྛ·ͨ͸εεΩ૲஍͔Βͷڑ཭͸21mɻ000353ͱ 000361ͷڞ௨఺:ՖͷܗଶͷΈ͔ΒιϝΠϤγϊͱ൑அ͠·͕ͨ͠,౰஍Ͱ͸ެԂͳͲʹ͍ΘΏΔατβΫϥͷෳ਺ ඼छΛࠞ২͢Δ͜ͱ͕͋ΔͷͰ,ՄೳͰ͋Ε͹ը૾(000353_4)͔Β඼छͷ֬ೝΛ͓ئ͍͠·͢ɻࢼྉ࠾औ஍͸ࣛࣇ ౡࢢ಺ɻḈຎ൒ౡ౦ଆͷࣛࣇౡ࿷ʹ໘͠ɺՐࡅྲྀ෾ग़෺͕ଯੵͯ͠Ͱ্͖ͨϊݪ୆஍্ɻඪߴ90mɻ1978೥ʹ଄ ੒͞Εͨॅ୐஍֎ԑͷެԂʹ২࠿͞Εͨݸମɻ২࠿஍ͷެԂͷ๺౦ଆͱ๺੢ଆʹंಓΛִͯͯ,֓Ͷೋ֊ՈҰށݐ ͯͷॅ୐஍͕͋Γ·͢ɻ૬ରর౓100%ɻंಓ͓ΑͼंಓԊ͍ͷ֗౮͔Βͷڑ཭͸΄΅ಉ͡ɻࣛࣇౡ஍ํؾ৅୆෇ ۙͷԹྔࢦ਺͸140લޙɻௐࠪ஍͸ඪߴ͕80mఔ౓ߴ͍ͷͰͦΕΑΓΘ͔ͣʹ௿͍ͱߟ͑ΒΕ·͢ɻஆԹଳͷর༿ थྛଳʹ૬౰͠·͕͢,ஆԹଳͷর༿थྛଳʹ૬౰͠·͕͢,২࠿஍ͷެԂͷೆ੢ଆͷࣼ౓30°ͷ֑஍ͷࣼ໘ʹৗ྘ ޿༿थΛओͱ͢Δೋ࣍ྛ·ͨ͸૲஍͕࢒ଘ͍ͯ͠Δ͚ͩͰ͢ɻ࿈೔׆ൃʹ෾Ր͍ͯ͠Δࡩౡত࿨Րޱ͔Βͷڑ཭͸ ͓Αͦ15kmɻ෩޲͖ʹΑͬͯ͸߱փ͕͋Γ·͢ɻҰൠʹࣛࣇౡ࿷पลͰ͸ۭத࣪౓ͷߴ͞ͱՐࢁփͷ෇ணʹΑΓ थ໦ͷװʹணੜ২෺͕ੜҭ͠΍͍͢܏޲͕͋Γ·͢ɻαϯϓϧͷՖห্ʹ΋Րࢁփ͋Δ͍͸ԫ࠭ͱࢥΘΕΔԚΕ͕ ΈΒΕ·͢ɻҰൠతʹ,Ұ੪ʹ࡙͍ͯҰ੪ʹࢄΔؔ౦ͱൺ΂Δͱ,౰஍Ͱ͸1ຊͷथͷ։Ֆظ͕ؒ௕͍(ݸʑͷՖ͕ॱ ࡙͍࣍ͯ,ॱ࣍ࢄΔ)܏޲͕͋ΔΑ͏Ͱ͢ɻ্هͷ։Ֆޙͷ೔਺͸αϯϓϦϯάͨ͠ʮՖʯͷ։Ֆ೔਺Ͱ͢ɻҢ౓ܦ ౓ʹ͍ͭͯ͸GPSઐ༻ػͷ৘ใ(ը૾000353_2Լஈ)Λೖྗ͠·ͨ͠ɻ $BTFJOEFUBJM ྔ͸ଟ͍͕ɺػցՄಡੑ͸௿͍

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هड़ͷίετWTղੳ࣌ͷॊೈੑ ϝλσʔλͷ࣭ͷ޲্͕EBUBESJWFOTDJFODFͷ伴ΛѲ͍ͬͯΔ

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• ܭଌٕज़ͷਐา͕Պֶͷख๏Λม͑Δ • σʔλۦಈܕՊֶͱͳͬͨੜ෺ֶ͸ܭࢉػՊֶͱ༥߹͢Δ • ஝ੵͨ͠σʔλ͕ҖྗΛൃش͢Δ࣌୅ͦ͜σʔλͷهड़͕ॏཁ • ͍͔ʹ࣭ͷߴ͍σʔλΛऩू͢Δ͔ %

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Պֶͱ͸هड़͢Δ͜ͱ ॻ͖ͱͲΊΑɻٞ࿦ͨ͜͠ͱ͸෩ͷதʹਧ͖ඈ͹ͯ͠͸͍͚ͳ͍

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& ୈ࢛ճNGSݱ৔ͷձ اըνʔϜ ͓ՖݟϓϩδΣΫτࢀՃऀͷΈͳ͞· ೔ຊͷσʔλϕʔεΛࢧ͑Δ DBCLS/NBDC/DDBJ ͷΈͳ͞· Bio OSSίϛϡχςΟ BioHackathon/SPARQLthon/PitagoraNetwork ͷΈͳ͞·

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' • ഑ྻղੳͷڭՊॻతͳॻ੶ • Dr. Bonoͷੜ໋Պֶσʔλղੳ (ϝσΟΧϧαΠΤϯε Πϯλʔφγϣφϧ) • NGSͷ࣮ݧख๏΍ղੳͷํ๏ʹ͍ͭͯ஌ΔͨΊͷॻ੶ • ࣍ੈ୅γʔΫΤϯεղੳελϯμʔυʙNGSͷϙςϯ γϟϧΛ׆͔͖͠ΔWET&DRY (༽౔ࣾ) • ౷ܭ΍ػցֶशͷଆ໘͔ΒόΠΦΠϯϑΥϚςΟΫεΛ஌ Γ͍ͨਓ޲͚ • ੜ໋৘ใॲཧʹ͓͚Δػցֶश ଟॏݕఆͱਪఆྔઃܭ (ػցֶशϓϩϑΣογϣφϧγϦʔζ) (ߨஊࣾ) • ࠷৽ͷݚڀൃද΍ߨԋΛYouTubeͰݟΔ • ౷߹TV http://togotv.dbcls.jp/ja/lecture.html • εϥΠυ • speakerdeck.com/inutano