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Assaf Zaritsky Decoupling global biases and local interactions between cell biological variables

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Quantifying protein-protein co-localization Dunn et al. (2011) Red Green Merge Condition B Condition A

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What additional information is hidden in co-localization data?

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Co-orientation of intracellular cytoskeletal networks in migrating cells

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Vimentin provides a structural template for microtubule growth Gan, Ding and Burckhardt et al. (2016) Genome-edited Retinal Pigment Epithelial (RPE) cells

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A relation between cell polarity and vimentin-microtubule interaction? Zhuo Gan

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Polarity-independent interaction of vimentin and microtubules ?

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Polarity-independent interaction of vimentin and microtubules

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What do we want to achieve? • Simultaneous investigation of mechanisms that drive global bias and local interactions How? • By modeling the observed agreement between matched variables as the cumulative global and local components Observed colocalization = Global bias + Local interaction

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DeBias

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DeBias

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DeBias

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DeBias

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DeBias

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DeBias

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Polarity-independent interaction of vimentin and microtubules

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Polarity-independent interaction of vimentin and microtubules Global index Local index

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Global bias: cell polarity

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Inferring co-localization and predicting dynamics from fixed cells during clathrin-mediated endocytosis (CME)

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Maturation of a clathrin coated pit Marcel Mettlen

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Cross-talk between signaling receptors (AKT) and components of the endocytic machinery Carlos Reis, H1299 cells

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Inferring co-localization and predicting dynamics from fixed cells during clathrin-mediated endocytosis (CME) Global index Local index

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Global bias: reduced TfnR in CCPs upon Akt inhibition

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More CCPs containing less TfnR alter CCPs dynamics upon AKT inhibition Reduced TfnR in CCPs upon Akt inhibition increased short-lived, (most likely) abortive events  decrease in CME efficiency Live imaging Internalization Fixed imaging

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Global bias: more CCPs with less TfnR upon Akt inhibition

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Spatio-temporal co-localization of Rac1 and Vav1 activity in a migrating cell (With Dan Marston, UNC)

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Resources Webserver: https://debias.biohpc.swmed.edu/ Source code: https://github.com/DanuserLab/DeBias https://elifesciences.org/content/6/e22323

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Take home message DeBias enables identifying the gorilla Simons and Chabris (1999)

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Uri Obolski, (Theory) Carlos Reis (Endocytosis) Zhuo Gan, (Vimentin, PKC) Yi Du (Webserver) Gaudenz Danuser Liya Ding Liqiang Wang Tamal Das Joachim Spatz Christoph Burckhardt Acknowledgments Sandy Schmid

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Thank you! https://elifesciences.org/content/6/e22323