3. 細胞膜を介した物質輸送

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νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ

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Eukaryotic cell 10 nm Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ νϟωϧͱτϥϯεϙʔλʔ

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Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ νϟωϧͱτϥϯεϙʔλʔ

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Mariana Ruiz, https://ja.wikipedia.org/wiki/ྲྀಈϞβΠΫϞσϧΑΓҾ༻ɺฤू Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ νϟωϧͱτϥϯεϙʔλʔ

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6 0 ~100 P O O O O CH2 CH CH2 O O C C O O ࢀߟɿDavid Sadavaଞஶ Life: The Science of Biology νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ

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ࢀߟɿDavid Sadavaଞஶ Life: The Science of Biology 6 0 ~100 P O O O O CH2 CH CH2 O O C C O O : νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ

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) : O2 CO2 N2 ࢀߟɿࡉ๔ͷ෼ࢠੜ෺ֶୈ6൛ H2O H+, Na+, HCO3 -,  K+, Ca2 +, Cl-, Mg2 +, Zn2 + νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ

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: : ATP Na+ ࢀߟɿyakugaku Lab https://yakugakulab.info/୯७֦ࢄɺଅਐ֦ࢄɺೳಈ༌ૹɺບಈ༌ૹ/ : : νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ

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ࢀߟɿDavid Sadavaଞஶ Life: The Science of Biology or νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ

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ࢀߟɿDavid Sadavaଞஶ Life: The Science of Biology or νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ

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Mariana Ruiz, https://ja.wikipedia.org/wiki/ྲྀಈϞβΠΫϞσϧΑΓҾ༻ɺฤू νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ

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ాதޫҰɺτϥϯεϙʔλʔͷ෼ྨͱݚڀ࢙ ΑΓҾ༻ Mariana Ruiz, https://ja.wikipedia.org/wiki/ྲྀಈϞβΠΫϞσϧΑΓҾ༻ɺฤू Ȃ6ʣ =ท+$01: χϯά͞ ϙʔλʔͷݸମϨϕϧͰͷػೳղੳ͕ߦΘΕɼͦΕΒͷҟ ৗʹΑΔපଶղ໌͕ਐߦதͰ͋Δɽ ɹਆܦ఻ୡ෺࣭ͷதͰɼάϧλϛϯࢎʹؔͯ͠͸ɼτϥϯ εϙʔλʔΛؚΊ೔ຊਓݚڀऀͷߩݙ͕େ͖͍ɽ1954 ೥ ਆܦՊֶڭत ࡉ๔֎ ࡉ๔಺ ࡉ๔֎ ࡉ๔಺ νϟωϧ τϥϯεϙʔλʔ ดঢ়ଶ ։ঢ়ଶ ֎޲͖ߏ଄ ಺޲͖ߏ଄ ਤ 2 ɹνϟωϧͱτϥϯεϙʔλʔͷ༌ૹػংͷҧ͍ νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ

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︎ : pore ︎ νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ ︎ ︎ ︎

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: ︎ ︎ ︎ ︎ ︎ ︎ ︎ SLC ATP ︎ ︎ ︎ ︎ ︎ ︎ ︎ ︎ ︎ CFTR ABC ︎ ︎ ︎ ATP ATP︎ ︎ 3 ࢀߟɿ ాதޫҰɺτϥϯεϙʔλʔͷ෼ྨͱݚڀ࢙ Solute carrier; SLC ATP-binding cassette; ABC ABC νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ

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SLC 3 νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ Sym-: Ұॹʹ

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ࢀߟɿઙ໺஌Ұ࿠ɼத௡༞հɺNa ڞ໾ܕάϧίʔε༌ૹମ(SGLT)ͱ͸ʙൃݟͷྺ࢙ͱੜཧػೳ GLUT SGLT Na GLUT SGLT Na Sodium-glucose cotransporter Facilitative glucose transporter νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ

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SGLT 1 SGLT1 ︎ ︎ 2 SGLT2 ︎ 3 SGLT3 ︎ νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ

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SGLT2 SGLT2͕ൃݱ SGLT1͕ൃݱ νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ

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SGLT2 νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ

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SGLT2

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ࢀߟɿSGLT2્֐ༀͱ͸ ݂ӷதͷάϧίʔε͕ݮগ ݂౶஋ͷ௿Լ ೘தʹഉᔔ͞ΕΔ άϧίʔε͕૿Ճ ൃ૝. τϥϯεϙʔλʔͷ׆ੑΛ੍ޚ͢Δ͜ͱͰɺ݂தͷ άϧίʔεೱ౓ΛௐઅͰ͖ΔͷͰ͸ʁ Na+ K+ SGLT2 GLUT2 Na+/K+ pump SGLT2 ( ) ( ) SGLT2 νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ

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ิ଍. λϯύΫ࣭ͷ౶Խͱ౶೘පͷපଶ (Complications of diabetes) D- OH OH OH HO H H H H C H O CH2 OH ︎ B III νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ Ў Ў

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LAT1 12 ࢀߟɿӬ৿ ᏅࢤɼۚҪ ޷ࠀ ͕ΜͱΞϛϊࢎτϥϯεϙʔλʔ LAT1 : L-type amino acid transporter 1. CD98hcλϯύΫ࣭ͱೋྔମΛܗ੒ɻ ( ) νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ

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LAT1 12 ͕Μࡉ๔Ͱൃݱྔ͕ ্ঢ͍ͯ͠Δ = : L-type amino acid transporter 1. CD98hcλϯύΫ࣭ͱೋྔମΛܗ੒ɻ LAT1 νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ ࢀߟɿӬ৿ ᏅࢤɼۚҪ ޷ࠀ ͕ΜͱΞϛϊࢎτϥϯεϙʔλʔ ( )

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LAT1 LAT1 : L-type amino acid transporter 1. CD98hcλϯύΫ࣭ͱೋྔମΛܗ੒ɻ νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ ࢀߟɿӬ৿ ᏅࢤɼۚҪ ޷ࠀ ͕ΜͱΞϛϊࢎτϥϯεϙʔλʔ ( )

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LAT1 LAT1 : L-type amino acid transporter 1. CD98hcλϯύΫ࣭ͱೋྔମΛܗ੒ɻ LAT1 LAT1 νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ ࢀߟɿӬ৿ ᏅࢤɼۚҪ ޷ࠀ ͕ΜͱΞϛϊࢎτϥϯεϙʔλʔ ( )

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νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ

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1. 2. : : (Liposome) Lipo-: -some: Proteoliposome reconstitution νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ

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3. A. B. C. ? ? ? A B A B C νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ

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WT: Wild-type A VS νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ

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1 ︎ ︎ 2 ︎ ︎ ︎ ︎ ︎ ︎ ︎ ︎ ︎ νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ

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︎ ︎ νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ

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Na+ Na+ K+ Na+-K+ νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ

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ࢀߟɿ େࡕେֶେֶӃҩֶܥݚڀՊɹࡉ๔ֶݚڀࣨHP DNA RNA νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ

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︎ ︎ Na+ ︎ ਆܦ఻ୡͷௐ੔ʹॏཁͳSLCτϥϯεϙʔλʔ νϟωϧͱτϥϯεϙʔλʔ (5-hydroxytryptamine; 5-HT) L- Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ

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ਆܦ఻ୡͷௐ੔ʹॏཁͳSLCτϥϯεϙʔλʔ νϟωϧͱτϥϯεϙʔλʔ Selective Serotonin Reuptake Inhibitor SSRI Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ

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σΠϏουɾαμϰΝ ஶɹؙࢁɹܟɺੴ࡚ɹହथ ༁ ΧϥʔਤղɹΞϝϦΧ൛ɹେֶੜ෺ֶͷڭՊॻɹୈ̍רɹࡉ๔ੜ෺ֶ David Sadava, David Hillis, H Heller ஶ Life: The Science of Biology Bruce Alberts, Julian Lewis, Martin Raff, Peter Walter, Keith Roberts, Alexander Johnson Molecular Biology of the Cell 5th edition ౦ژେֶੜ໋ՊֶڭՊॻฤूҕһձ ཧܥ૯߹ͷͨΊͷੜ໋Պֶ ୈ5൛ʙ෼ࢠɾࡉ๔ɾݸମ͔Β஌Δ“ੜ໋"ͷ͘͠Έ νϟωϧͱτϥϯεϙʔλʔ Ⅲ. ࡉ๔ບΛհͨ͠෺࣭༌ૹ Ӭ৿ ᏅࢤɺۚҪ ޷ࠀ ͕ΜͱΞϛϊࢎτϥϯεϙʔλʔ http://www.jbsoc.or.jp/seika/wp-content/uploads/2015/01/86-03-06.pdf ాதޫҰ τϥϯεϙʔλʔͷ෼ྨͱݚڀ࢙ http://www.tmd.ac.jp/mri/aud/resources/CLINN1.pdf