Tweet
Tweet

Slide 1

Slide 1 text

ςϯιϧ෼ղʹΑΔ౶೘පͷ ૊৫ಛҟతҨ఻ࢠൃݱͷ ౷߹ղੳΛ༻͍ͨؔ࿈࣬ױͷ༧ଌ (Taguchi et al., Genes 2022, 13(6), 1097) Y.-H. Taguchi, Turki Turki தԝେֶ ཧ޻ֶ෦ ෺ཧֶՊ Ωϯάα΢υେֶ ίϯϐϡʔλՊֶՊ 2025 ೥݄̏̒೔ Taguchi & Turki (MDPI) SIGBIO81 2025 ೥݄̏̒೔ 1 / 18

Slide 2

Slide 2 text

εϥΠυ֓ཁ 1 ݚڀഎܠͱ໨త 2 ςϯιϧ෼ղͷجૅ 3 HOSVD ղੳख๏ͱσʔλ 4 ݁Ռͷ֓ཁ 5 ߟ࡯ͱԠ༻Մೳੑ 6 ݁࿦ͱࠓޙͷల๬ 7 ࢀߟจݙ Taguchi & Turki (MDPI) SIGBIO81 2025 ೥݄̏̒೔ 2 / 18

Slide 3

Slide 3 text

ݚڀഎܠʢ1/2ʣ ౶೘ප͸ੈքతʹਂࠁͳ݈߁՝୊Ͱ͋Γɺਆܦม ੑ࣬ױ΍ඇΞϧίʔϧੑࢷ๱؊ɺ৺݂؅ো֐ͳͲ ଟ༷ͳ߹ซ঱ʢ༨පʣͱͷؔ࿈͕ࢦఠ͞Ε͍ͯΔɻ ͜͏ͨ͠༨පΛแׅతʹ೺Ѳɾ༧ଌ͢Δ͜ͱ͸ɺ ༧๷ҩֶ΍ݸผԽҩྍͷ؍఺͔Βॏཁɻ Ҩ఻ࢠൃݱσʔλΛ༻͍ͨղੳ͕ɺ࣬ױͷ෼ࢠج ൫Λղ໌͢Δ༗ྗͳखஈͱͯ͠஫໨͞Ε͍ͯΔɻ Taguchi & Turki (MDPI) SIGBIO81 2025 ೥݄̏̒೔ 3 / 18

Slide 4

Slide 4 text

ݚڀഎܠʢ2/2ʣ ैདྷ͸୯Ұ૊৫Ͱͷࠩ෼ղੳʢt ݕఆ, SAM, limma ౳ʣ͕ओྲྀ͕ͩɺશ਎ੑ࣬ױͷશମ૾Λे ෼ʹ͸ଊ͖͑Εͳ͍Մೳੑ͕͋Δɻ ຊݚڀͰ͸ɺςϯιϧ෼ղ (TD) Λ༻͍ͯϚϧν ૊৫Ҩ఻ࢠൃݱσʔλΛಉ࣌ʹղੳ͠ɺ౶೘පʹ ෇ਵ͢Δ࣬ױʢ༨පʣΛਪఆ͢Δɻ ໨తɿ୯Ұ૊৫ղੳͱൺֱ͠ͳ͕Βɺ౷߹ղੳͷ ϝϦοτΛݕূ͢Δͱͱ΋ʹɺ৽ͨͳ࣬ױؔ࿈৘ ใΛൃ۷͢Δɻ Taguchi & Turki (MDPI) SIGBIO81 2025 ೥݄̏̒೔ 4 / 18

Slide 5

Slide 5 text

ςϯιϧ෼ղʢTDʣͱ͸ ςϯιϧɿଟ࣍ݩ഑ྻɻҨ఻ࢠ ʷ ૊৫ ʷ ࣌ؒ ʷ ॲஔ ʜͳͲɺ3 ࣍ݩΛ௒͑Δσʔλߏ଄ɻ HOSVD (Higher-Order SVD)ɿߴ࣍ݩͷಛҟ஋ ෼ղʹΑΓɺίΞςϯιϧͱ֤Ϟʔυͷಛҟ஋ϕ Ϋτϧ΁෼ղ͢Δɻ ڭࢣͳ͠ख๏Ͱ͋Γɺෳ਺৚݅ɾ૊৫Λಉ࣌ʹ ѻͬͯ΋৘ใΛଛͳΘͣɺજࡏతͳมಈཁҼΛந ग़Մೳɻ ϙΠϯτ ୯Ұ૊৫͝ͱͷղੳͰ͸ݟ͑ͮΒ͍ʮԣஅతͳಛ௃ʯ Λଊ͑ΒΕΔͷ͕ڧΈɻ Taguchi & Turki (MDPI) SIGBIO81 2025 ೥݄̏̒೔ 5 / 18

Slide 6

Slide 6 text

HOSVDղੳϑϩʔʢ֓೦ਤʣ ʢ࿦จຊฤ Figure 1 ͳͲͷղੳύΠϓϥΠϯΛ૝ఆʣ 1) Ϛϧν૊৫σʔλΛߴ࣍ݩςϯιϧͱͯ͠ߏங 2) HOSVD Ͱ෼ղ ˠ ಛҟ஋ϕΫτϧ͔ΒมಈྔେͷҨ఻ࢠ Λબ୒ 3) બ୒Ҩ఻ࢠΛΤϯϦονϝϯτղੳ͠ɺؔ࿈࣬ױɾܦ࿏ Λ୳ࡧ Taguchi & Turki (MDPI) SIGBIO81 2025 ೥݄̏̒೔ 6 / 18

Slide 7

Slide 7 text

σʔλͱղੳͷྲྀΕ Ϟσϧಈ෺ɿϥοτʢ౶೘පϞσϧ vs. ରরʣ3 ૊৫ʢ؊ଁɺے೑ɺࢷ๱ʣʷ ෳ਺ि ʷ 2 ܥ౷ ˠ 6 ࣍ݩԽ ςϯιϧߏஙɿҨ఻ࢠ (∼ 31k) ʷ ि ʷ ݸମ ʷ ॲஔ (౶೘ප/ରর) ʷ ܥ౷ ʷ ૊৫ HOSVD Λద༻͠ɺओཁมಈ੒෼Λநग़ มಈྔ͕େ͖͍Ҩ఻ࢠʢ໿ 2542 ϓϩʔϒʣΛબ୒ ˠ Enrichr ౳Ͱػೳ෋ूղੳ Taguchi & Turki (MDPI) SIGBIO81 2025 ೥݄̏̒೔ 7 / 18

Slide 8

Slide 8 text

HOSVDͷ݁ՌʢҰྫɿfigure3.jpgʣ ಛҟ஋ϕΫτϧͷՄࢹԽʹΑΓɺͲͷϞʔυʢ࣍ݩʣʹେ͖ ͳมಈ͕͋Δ͔೺Ѳ͠΍͍͢ɻ ૊৫ԣஅతͳओཁ࣠ΛಛఆͰ͖Δͷ͕ TD ͷڧΈɻ Taguchi & Turki (MDPI) SIGBIO81 2025 ೥݄̏̒೔ 8 / 18

Slide 9

Slide 9 text

݁Ռʢ1/4ʣ ɿղੳख๏ൺֱ ղੳख๏ ༗ҙҨ఻ࢠ਺ ے೑༝དྷ උߟ ఏҊ (TD) ∼2542 ଟ਺ ౷߹ղੳ SAM (૊৫ผ) ਺ඦఔ౓ গ਺ ૊৫୯ಠ limma (૊৫ผ) ਺ेఔ౓ 2 ΄Ͳ ૊৫୯ಠ ComBat + ղੳ ਺ඦఔ౓ গ਺ όονิਖ਼ TD ͸ैདྷख๏ΑΓ޿ൣғͳҨ఻ࢠΛݕग़͠ɺے೑ͰͷมಈΛଊ͑΍ ͍͢ɻ ౷߹ղੳͳΒͰ͸ͷ༏Ґੑ͕ࣔࠦ͞ΕΔɻ Taguchi & Turki (MDPI) SIGBIO81 2025 ೥݄̏̒೔ 9 / 18

Slide 10

Slide 10 text

݁Ռʢ2/4ʣ ɿϕϯਤʹΑΔॏෳൺֱ ʢ࿦จதϕϯਤྫɿ૊৫ผղੳ DEGs ͱ TD બ୒Ҩ఻ࢠͷൺֱʣ Taguchi & Turki (MDPI) SIGBIO81 2025 ೥݄̏̒೔ 10 / 18

Slide 11

Slide 11 text

݁Ռʢ3/4ʣ ɿؔ࿈࣬ױͷΤϯϦονϝ ϯτ Taguchi et al., Genes 2022, 13(6), 1097, Figure 2 (ྫ) Taguchi & Turki (MDPI) SIGBIO81 2025 ೥݄̏̒೔ 11 / 18

Slide 12

Slide 12 text

݁Ռʢ4/4ʣ ɿؔ࿈࣬ױͷΤϯϦονϝ ϯτ நग़ͨ͠Ҩ఻ࢠ܈ͷΤϯϦονϝϯτղੳͰɺ౶೘පੑ৺ ے঱ͷ΄͔ɺϓϦΦϯප, ύʔΩϯιϯප, ΞϧπϋΠϚʔ ප౳্͕Ґώοτɻ ౶೘පͱਆܦมੑ࣬ױͷؔ࿈ੑΛ෼ࢠϨϕϧͰࣔࠦ͠ɺے ೑૊৫ͷҨ఻ࢠมಈ͕ؔ༩͍ͯ͠ΔՄೳੑ͕ු্ɻ ैདྷͷ୯Ұ૊৫ղੳͰ͸ಘΒΕͳ͍༨පͷख͕͔ΓΛಘΒ ΕΔ఺͕ TD ͷڧΈɻ Taguchi & Turki (MDPI) SIGBIO81 2025 ೥݄̏̒೔ 12 / 18

Slide 13

Slide 13 text

ߟ࡯ɿ౶೘පͱਆܦมੑ࣬ױͷؔ࿈ ౶೘ප͸ΞϧπϋΠϚʔප΍ύʔΩϯιϯපͳͲ ਆܦมੑ࣬ױͷϦεΫҼࢠͱ͞ΕɺઌߦݚڀͰ΋ ୅ँɾԌ঱ɾϛτίϯυϦΞػೳͷؔ࿈͕ࣔࠦ͞ Ε͍ͯΔɻ ຊݚڀͷ TD ղੳͰநग़͞ΕͨҨ఻ࢠ܈͸ɺ͜͏ ͨ͠୅ँܦ࿏΍ਆܦػೳʹ͔͔ΘΔ෼ࢠΛଟ͘ ؚΉɻ ౷߹ղੳͳΒͰ͸ͷ޿ҬతͳҨ఻ࢠબൈ͕ɺ෼ࢠ ج൫ͷڞ௨ੑΛ۩ମతʹࣔͨ͠ҙٛ͸େ͖͍ɻ Taguchi & Turki (MDPI) SIGBIO81 2025 ೥݄̏̒೔ 13 / 18

Slide 14

Slide 14 text

Ԡ༻Մೳੑ ຊΞϓϩʔν͸౶೘පҎ֎ʹ΋ɺෳ਺࣬ױؒͷະ ஌ͷؔ࿈ੑΛൃ۷͢Δͷʹ༗ޮɻ େن໛ͳϚϧνΦϛΫεʢήϊϜ, τϥϯεΫϦ ϓτʔϜ, ϝλϘϩʔϜ ౳ʣʹର֦ͯ͠ுͰ͖ɺ ૑ༀඪత୳ࡧ΍ϦεΫ༧ଌʹԠ༻Մೳɻ γϯάϧηϧղੳ΍࣌ܥྻղੳͱ૊Έ߹ΘͤΔ͜ ͱͰɺΑΓৄࡉͳපଶਐߦͷ෼ࢠϝΧχζϜ͕໌ Β͔ʹͳΔ༨஍͕͋Δɻ Taguchi & Turki (MDPI) SIGBIO81 2025 ೥݄̏̒೔ 14 / 18

Slide 15

Slide 15 text

݁࿦ Ϛϧν૊৫ͷҨ఻ࢠൃݱΛςϯιϧ෼ղͰ౷߹ղ ੳ͢Δ͜ͱͰɺ౶೘පͷ༨පʢਆܦมੑ࣬ױͳ ͲʣΛ໢ཏతʹਪఆͰ͖ΔՄೳੑΛࣔͨ͠ɻ ैདྷख๏Ͱ͸ݟಀ͞ΕΔے೑૊৫ͷҨ఻ࢠมಈ ͕ɺਆܦมੑܦ࿏ͷΤϯϦονϝϯτʹد༩ɻ ౷߹ղੳ͸୯ҰղੳΑΓ΋޿͍ࢹ໺Ͱ࣬ױؔ࿈෼ ࢠΛநग़͠ɺ෼ࢠج൫ͷڞ௨ੑΛղ໌͢Δ্Ͱ༗ ྗͳख๏ͱͳΔɻ Taguchi & Turki (MDPI) SIGBIO81 2025 ೥݄̏̒೔ 15 / 18

Slide 16

Slide 16 text

ࠓޙͷల๬ ຊख๏ͷྟচԠ༻ɿώτױऀ༝དྷϚϧνΦϛΫε σʔλΛ౷߹͠ɺ༨පϦεΫධՁ΍ݸผԽ࣏ྍઓ ུ΁ܨ͛Δɻ ଞ࣬ױͷ૊Έ߹Θͤʢ͕Μʴ໔Ӹ࣬ױͳͲʣͰͷ ૬ޓؔ࿈ղੳʹ΋ల։Մೳɻ ࣌ܥྻ΍γϯάϧηϧϨϕϧͰͷղੳɺػցֶश ͱͷ࿈ܞͳͲɺଟ֯తΞϓϩʔνʹΑΓਫ਼៛Խ͕ ظ଴͞ΕΔɻ Taguchi & Turki (MDPI) SIGBIO81 2025 ೥݄̏̒೔ 16 / 18

Slide 17

Slide 17 text

ࢀߟจݙ I Taguchi, Y.-H.; Turki, T. Genes 2022, 13(6), 1097. https://www.mdpi.com/2073-4425/13/6/1097 Taguchi, Y.-H.; Turki, T. (Supplementary Materials), https://www.mdpi.com/article/10.3390/genes13061097/s1 Taguchi & Turki (MDPI) SIGBIO81 2025 ೥݄̏̒೔ 17 / 18

Slide 18

Slide 18 text

͝ਗ਼ௌ͋Γ͕ͱ͏͍͟͝·ͨ͠ ࣭͝໰͸͍͟͝·͔͢ʁ Taguchi & Turki (MDPI) SIGBIO81 2025 ೥݄̏̒೔ 18 / 18