of BRD2 contains an alternatively spliced exon. A. A cartoon portraying the ~180 bp region in intron 2 of BRD2 that is highly conserved between human, dog, mouse, rat, and opossum, adapted from the ECR Browser. The yellow bar indicates the alternative exon 2a (92 bp) in intron 2. The green column near exon 3 is a microsatellite (GT repeats). A vertical axis cut-off of 75% identity is utilized to visualize only the significant alignments. Note that the level of conservation in intron 2 is higher than in the two adjacent coding exons. B. RT-PCR of human brain RNA using primers located in exon 2 and exon 3 of human BRD2. The upper weaker band contains a 92 bp conserved region in intron 2 (exon 2a), indicating the existence of mRNAs containing the alternatively spliced exon. C. RT-PCR of RNA from constructs containing different length of the GT repeats using primers across exon 2 to exon 3. It shows that the proportion of the alternatively spliced transcript (top band) increases relative to the regularly spliced transcript (lower band) with the shortening of the GT repeats. Shang et al. Page 15 NIH-PA Author Manuscript NIH-PA Aut
iqgap1 153+105bp siRNA_CHX con luc 50 siRNA con_CHX luc_CHX MQ 28 30 28 29 PTBP1 • The expression of iqgap1 is maintained at low levels in healthy brain tissues, but in brain tumors it is increased 28 28 29 30