Playing with Magnetic Beads inside Lab on a Chip Systems

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Magnetic actuator for the control and mixing of magnetic bead- based reactions on-chip Miguel Berenguel Alonso Jornades Doctorals 2014 G B S Grup de Sensors i Biosensors

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Poster FP 1.4 [email protected]

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Magnetic Beads

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Magnetic Beads Control

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Magnetic Beads Functionalization Control

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X Magnetic Beads Functionalization Control Chemical Group DNA Antibody Other

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Magnetic Beads Functionalization Control X

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Magnetic Beads Functionalization Control Commercial X

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Magnetic Beads Functionalization Control Commercial X Great Potential in Analysis

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Lab on a Chip

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Lab on a Chip

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Lab on a Chip

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Lab on a Chip

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Reaction Chamber Lab on a Chip

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Reaction Chamber Lab on a Chip

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Detection Chamber Reaction Chamber Lab on a Chip

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Detection Chamber Reaction Chamber Lab on a Chip

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Fabrication Process Lab on a Chip

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Fabrication Process A Lab on a Chip

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Fabrication Process A B Lab on a Chip

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Fabrication Process A B C Lab on a Chip

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Fabrication Process A B C D Lab on a Chip

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Advantages Lab on a Chip Magnetic Beads

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Limitations Lab on a Chip Magnetic Beads

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How do I control the MBs within the Microfluidic Chip?

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Magnetic Actuator Magnet Assembly Rotation MB Movement Magnet Pairs

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Magnetic Actuator Magnetic Rotation Axis Magnets Beads

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Magnetic Actuator 0º

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Magnetic Actuator 90º 0º

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Magnetic Actuator 90º 180º 0º

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Magnetic Actuator 90º 180º 0º 270º

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Magnetic Actuator 270º 180º 90º 0º

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Magnetic Actuator Magnetic Rotation Axis Magnets Beads

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Magnetic Actuator Magnetic Rotation Axis Magnets Beads What kind of magnet(s)?

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Magnetic Actuator Dr. X. Granados Dr. J. Faraudo

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Magnetic Actuator Simulation

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Magnetic Actuator B Magnet Couple

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The actuator enhances the microfluidic immunoassay by actively mixing

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Immunoassay E. Coli O157:H7

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Immunoassay E. Coli O157:H7 Enterohemorragic strain

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Immunoassay E. Coli O157:H7 Enterohemorragic strain Present in food

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Immunoassay E. Coli O157:H7 Enterohemorragic strain Present in food

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Immunoassay E. Coli O157:H7 Enterohemorragic strain Present in food Commercial MBs

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Immunoassay

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Immunoassay Off-Chip

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Immunoassay

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ImmunoassayOn-Chip

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Immunoassay Detection System LED Microfluidc Chip Optical Filter Detector Insertion Port

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Immunoassay Detection System LED Microfluidc Chip Optical Filter Detector Insertion Port

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Immunoassay Rotation Speed

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Immunoassay Calibration Curves 1.0 0.8 0.6 0.4 0.2 0.0 Fluorescence (mV) 2.5x10 4 2.0 1.5 1.0 0.5 0.0 E. Coli O157:H7 (CFU/mL) '0 mm/s' '1,7 mm/s'

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Immunoassay Calibration Curves 1.0 0.8 0.6 0.4 0.2 0.0 Fluorescence (mV) 2.5x10 4 2.0 1.5 1.0 0.5 0.0 E. Coli O157:H7 (CFU/mL) '0 mm/s' '1,7 mm/s'

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Immunoassay Sensitivity Detection Limit 2,7-fold Enhancement 603 CFU/mL

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Immunoassay Sensitivity Detection Limit 2,7-fold Enhancement 603 CFU/mL

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To sum up...

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Analytical Potential

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Analytical Potential Magnetic Beads Lab on a Chip

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Analytical Potential Magnetic Beads Lab on a Chip Magnetic Actuator

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Analytical Potential Magnetic Beads Lab on a Chip Magnetic Actuator Detection E.Coli O157:H7 2,7-fold Sensitivity Enhancement Detection Limit 603 CFU/mL