Playing with Magnetic Beads inside Lab on a Chip Systems

Presentation held at the Jornades Doctorals 2014, organized by the PhD Chemistry Program and the Chemistry Department, Universitat Autònoma de Barcelona.

Transcript

1. Magnetic actuator for the control and mixing of magnetic bead- based reactions on-chip Miguel Berenguel Alonso Jornades Doctorals 2014 G B S Grup de Sensors i Biosensors
3. Poster FP 1.4 miguel.berenguel@uab.cat
6. Magnetic Beads
7. Magnetic Beads Control
8. Magnetic Beads Functionalization Control
9. X Magnetic Beads Functionalization Control Chemical Group DNA Antibody Other
10. Magnetic Beads Functionalization Control X
11. Magnetic Beads Functionalization Control Commercial X
12. Magnetic Beads Functionalization Control Commercial X Great Potential in Analysis
14. Lab on a Chip
15. Lab on a Chip
16. Lab on a Chip
17. Lab on a Chip
18. Reaction Chamber Lab on a Chip
19. Reaction Chamber Lab on a Chip
20. Detection Chamber Reaction Chamber Lab on a Chip
21. Detection Chamber Reaction Chamber Lab on a Chip
22. Fabrication Process Lab on a Chip
23. Fabrication Process A Lab on a Chip
24. Fabrication Process A B Lab on a Chip
25. Fabrication Process A B C Lab on a Chip
26. Fabrication Process A B C D Lab on a Chip
28. Advantages Lab on a Chip Magnetic Beads
29. Limitations Lab on a Chip Magnetic Beads
31. How do I control the MBs within the Microfluidic Chip?
33. Magnetic Actuator Magnet Assembly Rotation MB Movement Magnet Pairs
34. Magnetic Actuator Magnetic Rotation Axis Magnets Beads
35. Magnetic Actuator 0º
36. Magnetic Actuator 90º 0º
37. Magnetic Actuator 90º 180º 0º
38. Magnetic Actuator 90º 180º 0º 270º
39. Magnetic Actuator 270º 180º 90º 0º
40. Magnetic Actuator Magnetic Rotation Axis Magnets Beads
41. Magnetic Actuator Magnetic Rotation Axis Magnets Beads What kind of magnet(s)?
42. Magnetic Actuator Dr. X. Granados Dr. J. Faraudo
43. Magnetic Actuator Simulation
44. Magnetic Actuator B Magnet Couple
47. The actuator enhances the microfluidic immunoassay by actively mixing
49. Immunoassay E. Coli O157:H7
50. Immunoassay E. Coli O157:H7 Enterohemorragic strain
51. Immunoassay E. Coli O157:H7 Enterohemorragic strain Present in food
52. Immunoassay E. Coli O157:H7 Enterohemorragic strain Present in food
53. Immunoassay E. Coli O157:H7 Enterohemorragic strain Present in food Commercial MBs
54. Immunoassay
55. Immunoassay Off-Chip
56. Immunoassay
57. ImmunoassayOn-Chip
58. Immunoassay Detection System LED Microfluidc Chip Optical Filter Detector Insertion Port
59. Immunoassay Detection System LED Microfluidc Chip Optical Filter Detector Insertion Port
60. Immunoassay Rotation Speed
61. Immunoassay Calibration Curves 1.0 0.8 0.6 0.4 0.2 0.0 Fluorescence (mV) 2.5x10 4 2.0 1.5 1.0 0.5 0.0 E. Coli O157:H7 (CFU/mL) '0 mm/s' '1,7 mm/s'
62. Immunoassay Calibration Curves 1.0 0.8 0.6 0.4 0.2 0.0 Fluorescence (mV) 2.5x10 4 2.0 1.5 1.0 0.5 0.0 E. Coli O157:H7 (CFU/mL) '0 mm/s' '1,7 mm/s'
63. Immunoassay Sensitivity Detection Limit 2,7-fold Enhancement 603 CFU/mL
64. Immunoassay Sensitivity Detection Limit 2,7-fold Enhancement 603 CFU/mL
65. To sum up...
66. Analytical Potential
67. Analytical Potential Magnetic Beads Lab on a Chip
68. Analytical Potential Magnetic Beads Lab on a Chip Magnetic Actuator
69. Analytical Potential Magnetic Beads Lab on a Chip Magnetic Actuator Detection E.Coli O157:H7 2,7-fold Sensitivity Enhancement Detection Limit 603 CFU/mL
71. Thank You very much for your attention!