• This setup uses an excitation source to excite the sample in a quartz cuvette • Measures the emitted signal power by Photomultiplier tube (PMT) • Diffraction grating splits the emitted signal into different wavelengths, which is then focused by parabolic mirror into the detector (PMT) • Detector is positioned on a translational stage, controlled by a dc motor which helps to detect the signal at different angles i.e. different wavelengths • A simple GUI on python for user inputs (motor speed, travel distance, and repetitions)
~ $ 400 (PCR) • Joined PRI in a research position to design a low–cost thermal cycler in affiliation with the NAST • A fully functional PCR device was designed within three months which was later validated by an independent research lab • Results were found comparable with the commercial devices in terms of sensitivity Project 1: Fig 2: a) Thermocycler b) Gene Amplification Result
Contamination in Drinking Water Project 2: • Measures the fluorescence emitted by the bacterial proteins to identify bacterial contamination in drinking water • Uses single semiconductor photodiode to detect the signal, which is amplified by bespoke transimpedance amplifier • The amplified voltage is processed using ADC and displayed correlated values on OLED Fig 3: Different Versions of WAS
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