PHILIPPE CASTAGNONE-SERENO, BOB ROBBINS, PABLO CASTILLO & MANY MANY OTHERS @davelunt firstname.lastname@example.org speakerdeck.com/davelunt slides available data accession: PRJNA340324 Amir Szitenberg & Laura Salazar have carried out much of this work AMIR SZITENBERG, LAURA SALAZAR, VIVIAN BLOK, SOUMI JOSEPH, DOMINIK LAETSCH, VALERIE WILLIAMSON, MARK BLAXTER, DAVE LUNT ROOT KNOT NEMATODE COMPARATIVE GENOMES
genome copies predating speciation Phylogeny in each genome copy A/B is identical M. incognita M. javanica M. arenaria M. ﬂoridensis A B Orthologue CDS supermatrix ML tree RAxML colours are different species not reproductive system
and content unannotated Intergenic DNA Repetitive elements SSRs Transposons homozygous APOMICTS ARE HYPOTRIPLOIDS B A A1 A2 B A1 A2 B A1 A2 B & Outgroups Automict Apomict Apomict Apomict HYPOTRIPLOID = not full triploid, some parts of genome are diploid 70 70 10 Mb 10 80 80 60 60 40 60 60
copy A2 copy number 1 2 A1-A2 are ~100% identical to each other copy A copy B copy number 1 2 3% divergence in protein coding regions illustration of diversity at each diploid locus Some loci are diploid A,B, some triploid A1,A2,B illustration of diversity at each triploid locus hypotriploid = not full triploid, some parts of genome are diploid
of diversity at each triploid locus not all loci are triploid, diploid loci add to read depth 100 peak many loci have read depth 100 some loci have read depth 200 A1 + A2 from high quality PacBio genome copy A1 copy B copy A2 copy number 1 2 sequence read depth A + B
How many phylogenetically independent contrasts? Long term evolution effects Power? Taxonomically narrow systems? Null hypothesis? Experimenter degrees of freedom? Phylogenetically controlled design? gene conversion and parasexual processes Note to self: must not rant against other study designs
breeding system NEMATODA TRANSPOSABLE ELEMENTS FOLLOW PHYLOGENY Szitenberg et al Genome Biology & Evolution 2016 doi:10.1093/gbe/evw208 DNA TEs LTRs transposons 500 million years of evolution 42 genomes Analyses and scripts DOI: 10.5281/zenodo.58256
REPRODUCIBILITY Better science will be done, and more problems will be solved, with data freely and immediately available to all Open standards, sharing, & reproducible analysis pipelines will beneﬁt all @davelunt email@example.com speakerdeck.com/davelunt slides available data accession: PRJNA340324 both raw data and genome assemblies THANK YOU, QUESTIONS?