INDEAR WebServices - Bioinformatics

INDEAR

Transcript

1. Whole Genome Shotgun Sequencing Santiago Revale, Estefania Mancini and Martin P. Vazquez Genomics and Bioinformatics, INDEAR-CONICET, Rosario (2000), Santa Fe, Argentina A platform for delivery and analysis of Next-Generation Results Background The advent of high-throughput Next-Generation Sequencing (NGS) has created data-management challenges. Even if the data-volume issue is being resolved by both cheaper and increasingly innovative hardware solutions, the greater problem is managing the complexity of data. NGS data analysis requires more time, money and resources than that for data generation, becoming now the limiting step in genomic studies. INDEAR holds the first next-generation sequencer available in Argentina, a ROCHE 454 GS-FLX Titanium. According to the situation exposed before we have developed INDEAR WebServices, a user friendly platform for the analysis and delivery of NGS results. Results We have developed a platform capable of processing data from metagenomics, transcriptomics, de novo prokaryotic and eukaryotic genome sequencing, amplicon sequencing and reference mapping in diverse projects such as human clinical samples. In addition, INDEAR Webservices offers a set of user friendly bioinformatics GUI tools for graphical data browsing and interpretation allowing clients to visualize and interact with resulting data. INDEAR WebServices is accessed by a username and password and the data is maintained confidential. Conclusion Our main goal is to assist users with no bioinformatics background in the interpretation of NGS results. INDEAR WebServices offers a solution for researchers making the use of NGS accessible for a wider diversity of professionals. The platform is in continuous development. We are improving our services day by day in order to satisfy every new project requirement. Graphical Data Exploration. Download processed data with annotations and metabolic pathways. Filter your results grouping by function or subsystem. Perform BLAST search against your genome sequences. Amplicon Sequencing E.g.: 16S rRNA V4 region Ecological analysis. Interpretation of results using colorful graphics in two and three dimension. Run customized PANGEA HT pipeline in our servers. Receive an email when it has finished with the results. Perform BLAST search against your pyrotags database. ROCHE 454 GS-FLX Titanium Sequencing Bacterial genomes Total gDNA DNA obtained directly from complex samples (e.g., enviromental, human gut, viral infective variants, etc.) Acid mines Soils Polluted sediments Extreme environments INDEAR WebServices