Richard Hastings

Transcript

1. Dr Richard Hastings Technical Director BioCote Ltd
2. Novel study measures antiviral effect 2 Richard Hastings Microbiologist – University of Warwick Technical Director – BioCote Ltd
3. Or: What happens if you don’t catch ‘flu? 3
4. ‘Catch it. Bin it. Kill it.’ Campaign 4
5. So, what happens if you don’t catch, bin and kill ‘flu? 5
6. What else do we have in our anti-influenza arsenal? 6 Weak points?
7. 7 A little virology: Influenza virus showing haemagglutinin and neuraminidase spikes
8. 8 A real influenza virus (approx.100nm)
9. 9 How ‘flu catches you!
10. 10 Influenza is believed to be transmitted through the air by coughs or sneezes, creating aerosols containing the virus. How is ‘flu spread?
11. 11 Influenza can also be transmitted by direct contact with bird droppings and nasal secretions or through contact with contaminated surfaces. Airborne aerosols have been thought to cause most infections, although which means of transmission is most important is not absolutely clear Transmission of influenza A in human beings. Lancet Infect Dis 7 (4): 257–65. Brankston G, Gitterman L, Hirji Z, Lemieux C, Gardam M (April 2007).
12. 12 We know silver is antimicrobial
13. We know how silver works 13
14. • Can captured silver knock out virus protein sufficiently to inactivate the virus? • Can we measure such an effect? 14 Let’s explore something new! We already know: •Silver acts on protein •Viruses are largely protein •Silver is antiviral
15. The difficulty working with viruses 15
16. Study: Quantifying the antiviral property of BioCote®-treated polymeric and laminate materials Pneumovirus Laboratory, School of Life Sciences, University of Warwick 16 Paul Scott Tony Marriott Andrew Easton Richard Hastings
17. Step 1 17 Untreated BioCote®-treated Exposure of Influenza A virus strain H1N1 to test material Say 1 million viruses What happens in the lab?
18. Step 2 18 Recovery of virus from test material Infect laboratory cells with virus dilutions Serially dilute recovered virus Neat 10-1 10-2 10-3 10-4 10-5 What happens in the lab?
19. Step 3 19 Detect and count number of virus infecting cells Untreated BioCote®- treated Decreasing number of viruses What happens in the lab?
20. 20 What do the results look like? Uninfected cells Influenza virus infected cells
21. 21 1.00E+00 1.00E+01 1.00E+02 1.00E+03 1.00E+04 1.00E+05 1.00E+06 Log Reduction of Virus (log 10) Initial Amount of Virus Final Amount of Virus What do the results tell us?
22. Study Outcomes • Significant reductions of infectious virus compared to non-silver controls • Silver contained in solid materials exhibits an efficacious antiviral property • Investigate the wider antiviral effect - Norovirus? • Measurement of effect in situ 22