Presentation of a paper for the Nanotechnology course

Paper:
B. R. Lutz, P. Trinh, C. Ball, E. Fu, and P. Yager. “Two-dimensional paper networks: programmable fluidic disconnects for multi-step processes in shaped paper.” In: Lab Chip 11 (24 2011), pp. 4274–4278. DOI: 10.1039/C1LC20758J. URL: http://dx.doi.org/10.1039/C1LC20758J.

Course web page:
https://fenix.tecnico.ulisboa.pt/disciplinas/N3251795/2017-2018/1-semestre.

Transcript

1. Two-dimensional paper networks: programmable fluidic disconnects for multi-step processes in shaped paper Barry R. Lutz, Philip Trinh, Cameron Ball, Elain Fu and Paul Yager João Palmeiro | 79114 Nanotechnology
2. Why? Laboratory assays + Multi-step protocols = High performance!
3. Why? Laboratory assays + Multi-step protocols = High performance! But… Assays + Paper-based tests = Single fluidic step :’(
4. Why? Laboratory assays + Multi-step protocols = High performance! But… Assays + Paper-based tests = Single fluidic step :’( So… What if we could get them together?
5. Why? Multi-step laboratory assays
6. Why? Multi-step laboratory assays Simple point-of-care devices
7. Why? Multi-step laboratory assays Simple point-of-care devices High performance + Easy to use + Inexpensive!
8. How? Two basic functions: 1. Controlling the arrival time of reagents to a detection zone
9. How? Two basic functions: 1. Controlling the arrival time of reagents to a detection zone 2. Controlling the shut off time of each reagent
10. How? Two basic functions: 1. Controlling the arrival time of reagents to a detection zone 2. Controlling the shut off time of each reagent Timing mechanisms into paper devices “Program” automated multi-step assays
11. How? Two-dimensional paper networks (2DPNs)
12. How? Two-dimensional paper networks (2DPNs) Materials from lateral flow tests
13. How? Two-dimensional paper networks (2DPNs) Materials from lateral flow tests Multiple converging fluid inlets to control the arrival time Legs of different lengths
14. How? Two-dimensional paper networks (2DPNs) Materials from lateral flow tests Multiple converging fluid inlets to control the arrival time Legs of different lengths But…
15. How? How to control the shut off time of each reagent?
16. How? Single shared well as the fluid source!
17. 1. Fluidic disconnects in isolated strips Nitrocellulose legs were arranged on a cartridge
18. 1. Fluidic disconnects in isolated strips Each leg protruded a different length
19. 1. Fluidic disconnects in isolated strips Regulator to increase the rate of fluid depletion + to humidify
20. 1. Fluidic disconnects in isolated strips Fluid level dropped due to wicking + Each leg was disconnected
21. 1. Fluidic disconnects in isolated strips Fluid front position in each leg vs. Time
22. 1. Fluidic disconnects in isolated strips Lucas-Washburn equation for 1D capillary uptake (2 = )
23. 1. Fluidic disconnects in isolated strips Until each leg broke contact with the fluid in the well
24. 1. Fluidic disconnects in isolated strips Fluid front migrated due to relaxation into the dry membrane
25. 1. Fluidic disconnects in isolated strips Relative errors in shut off times between 5%-20%
26. 1. Fluidic disconnects in isolated strips Fluid delivery was shut off in a well- controlled and timed sequence
27. 1. Fluidic disconnects in isolated strips Drop rate of fluid was dominated by the regulator
28. 1. Fluidic disconnects in isolated strips Drop rate of fluid was dominated by the regulator It provides significant benefits: Differential between leg immersion depths to be larger Reduced impact of device fabrication errors
29. 1. Fluidic disconnects in isolated strips Drop rate of fluid was dominated by the regulator It provides significant benefits: Differential between leg immersion depths to be larger Reduced impact of device fabrication errors Decouples the design for shut off timing from the device design
30. 1. Fluidic disconnects in isolated strips Drop rate of fluid was dominated by the regulator It provides significant benefits: Differential between leg immersion depths to be larger Reduced impact of device fabrication errors Decouples the design for shut off timing from the device design To humidify the device to reduce evaporation and timing errors
31. 2. Fluidic disconnects in a 2DPN Fluid delivery in separate legs to be shut off at programmed times
32. 2. Fluidic disconnects in a 2DPN Connect the legs to control the arrival time to a common point
33. 2. Fluidic disconnects in a 2DPN Autonomous multi-step fluid delivery
34. 2. Fluidic disconnects in a 2DPN Single large cellulose absorbent pad:
35. 2. Fluidic disconnects in a 2DPN 1. Regulator
36. 2. Fluidic disconnects in a 2DPN 1. Regulator 2. Humidifier
37. 2. Fluidic disconnects in a 2DPN 1. Regulator 2. Humidifier 3. Downstream wicking pad
38. 2. Fluidic disconnects in a 2DPN
39. 2. Fluidic disconnects in a 2DPN
40. 2. Fluidic disconnects in a 2DPN
41. 2. Fluidic disconnects in a 2DPN
42. 2. Fluidic disconnects in a 2DPN
43. 2. Fluidic disconnects in a 2DPN
44. 2. Fluidic disconnects in a 2DPN Viscosity + Surface tension have linear impacts on the flowrate
45. 2. Fluidic disconnects in a 2DPN Viscosity + Surface tension have linear impacts on the flowrate Glass fiber pads used in some LFTs could reduce transients
46. 2. Fluidic disconnects in a 2DPN Viscosity + Surface tension have linear impacts on the flowrate Glass fiber pads used in some LFTs could reduce transients Uniform-width legs + Leg spacings
47. 2. Fluidic disconnects in a 2DPN Viscosity + Surface tension have linear impacts on the flowrate Glass fiber pads used in some LFTs could reduce transients Uniform-width legs + Leg spacings Shut-off method is compatible with paper devices fabricated by different 2D patterning methods (photoresist patterning)
48. Conclusions 2DPN + Volume-limited source = Autonomous multi-step!
49. Conclusions 2DPN + Volume-limited source = Autonomous multi-step! Multi-step lab assays into simple point-of-care devices!
50. Conclusions 2DPN + Volume-limited source = Autonomous multi-step! Multi-step lab assays into simple point-of-care devices! High performance + Easy to use!
51. Conclusions 2DPN + Volume-limited source = Autonomous multi-step! Multi-step lab assays into simple point-of-care devices! High performance + Easy to use! Multi-step + Branched paper devices = Multi-analyte detection!
52. Conclusions 2DPN + Volume-limited source = Autonomous multi-step! Multi-step lab assays into simple point-of-care devices! High performance + Easy to use! Multi-step + Branched paper devices = Multi-analyte detection! Future: Programming 2DPNs to carry out analytical tests!
53. What? Point-of-care platform for autonomous multi- step fluidic processing
54. Two-dimensional paper networks: programmable fluidic disconnects for multi-step processes in shaped paper Barry R. Lutz, Philip Trinh, Cameron Ball, Elain Fu and Paul Yager Thanks!