mesodermal tissue. • Blocks align down the anteroposterior axis flanking neural tube. • Cells in somite fated to become bone, muscle, or skin in trunk and tail segments. Bone Muscle Skin photo: www.animalpicturesarchive.com • First visible segmented structure in developing embryo.
& Xnot important in embryonic mesoderm patterning. • Xbra in particular is implicated in somitogenesis. • Previous research addresses embryonic expression. • When, and where are these genes expressed in post-embryonic tissues? Xbra: Xnot2:
and Xnot2 in sections. • Modify 12-101 protocol for use in H. scutatum. • Modify my protocol to examine expression of these genes in late stage Xenopus (larval and metamorphosis stages). • Clone the H. scutatum homolog of these genes for in situ hybridization analysis of tail development in embryonic, larval, juvenile, and adult stages.