#tardigate: tardígrados y bioinformática en transferencia genética horizontal

Revisión de la controversia sobre el primer genoma de tardígrado. Preparado para el curso electivo en Transferencia Genética Horizontal 2016 de la EP Genética y Biotecnología UNMSM

Transcript

1. #tardigate TARDÍGRADOS y Bioinformática en TGH
2. #tardigate • Intro to tardigrade • On #tardigate • Consequences • Prokaryote to Eukaryote HGT? • Conclusions
3. Intro to tardigrade
4. #tardigate
5. Levin, M.,... & Fernandez-Valverde, S. L. (2016). The mid-developmental transition and the evolution of animal body plans. Nature.
6. #tardigate Goldstein lab – tardigrades: https://www.flickr.com/photos/waterbears/
7. #tardigate Goldstein lab – tardigrades: https://www.flickr.com/photos/waterbears/
8. #tardigate • September 20, 2016 12:05 PM EDT - The water bear, a tiny aquatic animal, can survive in many extreme conditions, including space. A dry water bear shows no signs of life, but a drop of water can bring it back to normal activity. (Daiki D. Horikawa) https://www.washingtonpost.com/video/national/watch-a-dried-up-water-bear-come-back-to-life/2016/09/20/7f202bf8-7f4d-11e6-ad0e-ab0d12c779b1_video.html
9. On #tardigate
10. #tardigate
11. #tardigate AF means "As F***" http://www.internetslang.com
12. #tardigate
13. #tardigate
14. #tardigate
15. #tardigate Boothby, T. C., Tenlen, J. R., Smith, F. W., Wang, J. R., Patanella, K. A., Nishimura, E. O., ... & Messina, D. N. (2015). Evidence for extensive horizontal gene transfer from the draft genome of a tardigrade. Proceedings of the National Academy of Sciences, 112(52), 15976-15981.
16. #tardigate Boothby, T. C., Tenlen, J. R., Smith, F. W., Wang, J. R., Patanella, K. A., Nishimura, E. O., ... & Messina, D. N. (2015). Evidence for extensive horizontal gene transfer from the draft genome of a tardigrade. Proceedings of the National Academy of Sciences, 112(52), 15976-15981.
17. #tardigate Boothby, T. C., Tenlen, J. R., Smith, F. W., Wang, J. R., Patanella, K. A., Nishimura, E. O., ... & Messina, D. N. (2015). Evidence for extensive horizontal gene transfer from the draft genome of a tardigrade. Proceedings of the National Academy of Sciences, 112(52), 15976-15981.
18. #tardigate Boothby, T. C., Tenlen, J. R., Smith, F. W., Wang, J. R., Patanella, K. A., Nishimura, E. O., ... & Messina, D. N. (2015). Evidence for extensive horizontal gene transfer from the draft genome of a tardigrade. Proceedings of the National Academy of Sciences, 112(52), 15976- 15981.
19. #tardigate Koutsovoulos, G., Kumar, S., Laetsch, D. R., Stevens, L., Daub, J., Conlon, C., ... & Blaxter, M. (2016). No evidence for extensive horizontal gene transfer in the genome of the tardigrade Hypsibius dujardini. Proceedings of the National Academy of Sciences, 113(18), 5053- 5058.
20. #tardigate Koutsovoulos, G., Kumar, S., Laetsch, D. R., Stevens, L., Daub, J., Conlon, C., ... & Blaxter, M. (2016). No evidence for extensive horizontal gene transfer in the genome of the tardigrade Hypsibius dujardini. Proceedings of the National Academy of Sciences, 113(18), 5053- 5058.
21. #tardigate Hashimoto, T., Horikawa, D. D., Saito, Y., Kuwahara, H., Kozuka-Hata, H., Shin, T., ... & Enomoto, A. (2016). Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein. Nature Communications, 7.
22. #tardigate Hashimoto, T., Horikawa, D. D., Saito, Y., Kuwahara, H., Kozuka-Hata, H., Shin, T., ... & Enomoto, A. (2016). Extremotolerant tardigrade genome and improved radiotolerance of human cultured cells by tardigrade-unique protein. Nature Communications, 7. Also: http://nematode s.org/blog/a-tr ue-tardigrade-g enome/
23. Consequences
26. • GUIDELINES: 1. Cutting-edge Bioinformatics 2. Environmental small-unit rDNA- based protocols 3. Sequencing of multiple genomes of same and related species. 4. Phylogenetic analysis 5. Syntheny analysis 6. Transcription (intron architecture)
27. #tardigate http://www.igs.umaryland.edu/labs/lgthgt/
28. http://www.igs.umaryland.edu/labs/lgthgt/
29. #tardigate
32. Conclusions
33. #tardigate
34. #tardigate
35. #tardigate http://nematodes.org/blog/eight-things-i-learnt-from-tardigate/
36. Terminó
37. Terminó