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Sequence Read Archive: Database for High-throug...

Sequence Read Archive: Database for High-throughput sequencing best practice 2012

「次世代シーケンス解析と公共データベース: Sequence Read Archiveを使い倒す」

Tazro Inutano Ohta

November 20, 2012
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  1. Ͳ͔͜ΒͰ΋ಉ͡σʔλʹΞΫηεՄೳ Data ID : 000001 organism : mouse cell :

    nervous cell sequencer : 454 date : 2011 12 08 >Seq_Numero_1 ATGCATGCATGCATG CATGCATGCATGCAT GCATGCATGCATGCA TGCATGCATGCATGC ATGCATGCATGCATG CATGATGCATGCATG CATGCATGCATGCAT GCATGCATGCATGCA TGCATGTGCATGTGC */4%$ σʔλަ׵ ഑ྻσʔλ ϝλσʔλΛొ࿥
  2. ొ࿥͞ΕͨσʔλͷγʔέϯεΫΦϦςΟΛݟΔ ˓ 43"ͷσʔλ͸ৗʹྑ͍΋ͷͱ͸ݶΒͳ͍ ˓ ϛεγʔέϯε΋ొ࿥͞Εσʔλ͕ೖ͍ͬͯΔ ˓ ৚͕݅ಉ͡σʔλͳΒਫ਼౓ͷྑ͍΋ͷΛ࢖͍͍ͨ ˓ 'BTU2$ʹΑͬͯશͯͷ43"σʔλͷΫΦϦςΟΛܭࢉ ˓

    IUUQXXXCJPJOGPSNBUJDTCBCSBIBNBDVLQSPKFDUTGBTURD ͝རӹ ˓ μ΢ϯϩʔυʹҰ൩͔͔ͬͨσʔλ͕յΕ͍ͯͨͱ͍͏൵ܶΛճආ ˓ γʔέϯεΫΦϦςΟͷൺֱΛ͢Δ͚ͩͰ΋ָ͍͠
  3. ΞʔΧΠϒ͸Ͳ͏͋Δ΂͖͔ ˓ σʔλެ։ͷෑډΛԼ͛Δ ˓ σʔλΛެ։͢ΔͨΊͷํ๏Λඪ४Խ͢Δ ˓ NJOJNVNJOGPSNBUJPOͳͲ ˓ ΑΓ؆୯ʹσʔλΛొ࿥͢ΔͨΊͷ࢓૊Έ ˓

    σʔλར༻Λଅਐ͢Δ ˓ ެ։σʔλΛΑΓ࢖͍΍͘͢੔ཧ͢Δ ˓ ެ։σʔλΛ༗ޮར༻͢ΔͨΊͷํ๏࿦ ˓ σʔλΛग़͢ਓʹ͝རӹΛ ˓ ެ։σʔλʹ%0*ΛৼͬͯҾ༻ΛՄೳʹ͢Δ౳