mRNA ワクチンのコードを読もう / Let's Read the mRNA Vaccine Code.

2021年9月19日(日) に開催したアカデミーキャンプ VR 2021.9「キミが探る！驚異の現代＆近未来医療」DAY 2 「mRNA ワクチンのコードを読もう」のスライドです。

Transcript

1. VR 2021.9 & DAY2 – mRNA ( ) VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.1/31
2. https://speakerdeck.com/ks91 VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.2/31
3. mRNA w w mRNA VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.3/31
4. by ( JM ) cord : ( ) code : ( ) ← ( ) chord : ( ) mRNA RNA VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.4/31
5. (( ) ) mRNA . . . VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.5/31
6. . . . DNA RNA DNA — bert hubert ← (↓ ) bert hubert ( ) “Reverse Engineering the source code of the BioNTech/Pfizer SARS-CoV-2 Vaccine” https://berthub.eu/articles/posts/reverse-engineering-source-code-of-the-biontech-pfizer-vaccine/ (↑ ) BioNTech/Pfizer https://note.com/yubais/n/n349ab986da42 ( SF ( ) ) “DNA: the code of life – Surprise: it is fully digital!” https://berthub.eu/dna/ ( ( ) ) ( mRNA ) VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.6/31
7. DNA RNA DNA ( ) ( ) ↓ (DNA RNA ) mRNA ( ) ( ) ↓ ( 3D ) ( ) VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.7/31
8. DNA ਫૉ ࢎૉ ஠ૉ ୸ૉ Ϧϯ — — "
   
   Ξσχϯ (
   
   άΞχϯ 5
   
   νϛϯ $
   
   γτγϯ : Wikipedia ( ) jurassic park velociraptor dna . . . CTT GAC AAA GTT GAG . . . DNA DNA 3 A T G C G C 1 2 VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.8/31
9. DNA (1) Ԙج σΦΩγ ϦϘʔε 5’ 3’ 5’ 5’ 3’ 4’ 3’ 2’ 1’ Ϧϯࢎ $ $ $ " " " 5 5 5 ( ( ( ຤୺ ຤୺ ຤୺ ຤୺ DNA = ( + ( ) + )* ( ) ( ) ′ ( ) DNA CG https://youtu.be/7Hk9jct2ozY?t=147 DNA/RNA (3′ → 5′ ) 5′ → 3′ ( ) 5′ → 3′ 3′ → 5′ VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.9/31
10. DNA (2) ` ` ` ` ` ` ` ` ࠯ͷૹΓํ޲ ઌߦ͢Δ࠯ ஗ΕΔ࠯ ࠯ͷૹΓํ޲ ෳ੡ϑΥʔΫ %/" ϙϦϝϥʔθ సࣸث Ԭ࡚ϑϥάϝϯτ  ೥ʹ༧૝͞Ε
     ೥ʹൃݟ ᶃ ᶄ ᶅ %/" ϙϦϝϥʔθ సࣸث ( 100 ) VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.10/31
11. DNA mRNA CG https://youtu.be/7Hk9jct2ozY?t=201 DNA 3′ → 5′ mRNA 5′ → 3′ RNA T ( ) U ( ) ← ∼ ← DNA mRNA GIF https://upload.wikimedia.org/wikipedia/commons/9/94/Protein_translation.gif RNA ( ) 3D 3D 3D 3D ( ) RNA VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.11/31
12. VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.12/31
13. mRNA bert hubert VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.13/31
14. mRNA WHO (World Health Organization) (bert hubert ) GitHub (← ) ( ) 500 ( : WHO) VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.14/31
15. mRNA 4284 1 30 RNA 1 1 , 000 1 1 , 000 , 000 RNA 1 0 . 53 × 10−21 2 ← 30 6 × 1016 RNA 14 ( ; 1 8 ) . . . 4284 RNA 13 1 7.5 VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.15/31
16. mRNA cap : ( ) 5′-UTR : 5′ ( ) (UnTranslated Region) sig : ( ) S protein_mut : 3′-UTR : 3′ ( ) poly(A) : ( ) VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.16/31
17. cap ( ) GA ← mRNA VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.17/31
18. mRNA cap : ( ) 5′-UTR : 5′ ( ) (UnTranslated Region) sig : ( ) S protein_mut : 3′-UTR : 3′ ( ) poly(A) : ( ) VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.18/31
19. 5′ GAAΨAAACΨAGΨAΨΨCΨΨCΨGGΨCCCCACAGACΨCAGAGAGAACCCGCCACC RNA A,C,G, U(T ) Ψ ( ) U 1-methyl-3′-pseudouridylyl 1- -3′- ( = (U)+ ); U RNA U Ψ 5′ VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.19/31
20. mRNA cap : ( ) 5′-UTR : 5′ ( ) (UnTranslated Region) sig : ( ) S protein_mut : 3′-UTR : 3′ ( ) poly(A) : ( ) VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.20/31
21. 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 3 Virus: AUG UUU GUU UUU CUU GUU UUA UUG CCA CUA GUC UCU AGU CAG UGU GUU Vaccine: AUG UUC GUG UUC CUG GUG CUG CUG CCU CUG GUG UCC AGC CAG UGU GUG ! ! ! ! ! ! ! ! ! ! ! ! ! ! Ψ U / S ( ) RNA → ⇒ 2 × 3 26 64 20 VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.21/31
22. G U A C U C A G U C A G U C A G U C A G U C A G U C A G U C A G U C A G U C A G U C A G U C A G U C A G U C A G U C A G U C A G U C A G U C A G U U U C C C A A A G G G 5´ 3´ 3´ 3´ 3´ Arg (R) Ser (S) Lys (K) Asn (N) Thr (T) Met (M) Ile (I) Arg (R) Gln (Q) His (H) Pro (P) Leu (L) Trp (W) Cys (C) Tyr (Y) Ser (S) Leu (L) Phe (F) Gly (G) Glu (E) Asp (D) Ala (A) Val (V) Start Stop : Wikipedia ← (3 1 ) Ala(A) : Cys(C): Asp(D): Glu(E) : Phe(F): Gly(G) : His(H) : Ile(I) : Lys(K) : Leu(L) : Met(M): Asn(N): Pro(P) : Gln(Q): Arg(R) : Ser(S) : Thr(T) : Val(V) : Trp(W): Tyr(Y) : G C RNA A♥T/U G ♥C G C VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.22/31
23. mRNA cap : ( ) 5′-UTR : 5′ ( ) (UnTranslated Region) sig : ( ) S protein_mut : 3′-UTR : 3′ ( ) poly(A) : ( ) VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.23/31
24. (1) * * L D K V E A E V Q I D R L I T G Virus: CUU GAC AAA GUU GAG GCU GAA GUG CAA AUU GAU AGG UUG AUC ACA GGC Vaccine: CUG GAC CCU CCU GAG GCC GAG GUG CAG AUC GAC AGA CUG AUC ACA GGC L D P P E A E V Q I D R L I T G ! !!! !! ! ! ! ! ! ! ! 3,777 . . . (* ) K( )-V( ) P( )-P( ) ( ) ( ) → VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.24/31
25. (2) V L K G V K L H Y T s Virus: GUG CUC AAA GGA GUC AAA UUA CAU UAC ACA UAA Vaccine: GUG CUG AAG GGC GUG AAA CUG CAC UAC ACA UGA UGA V L K G V K L H Y T s s ! ! ! ! ! ! ! ! ( s ) UAA 1 UGA 2 ( . . . ) VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.25/31
26. mRNA cap : ( ) 5′-UTR : 5′ ( ) (UnTranslated Region) sig : ( ) S protein_mut : 3′-UTR : 3′ ( ) poly(A) : ( ) VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.26/31
27. 3′ 5′ 3′ 3′ (Wikipedia) 3′ . . . 3′ RNA RNA VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.27/31
28. mRNA cap : ( ) 5′-UTR : 5′ ( ) (UnTranslated Region) sig : ( ) S protein_mut : 3′-UTR : 3′ ( ) poly(A) : ( ) VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.28/31
29. ( ) mRNA AAAAAAAAAAAAAAAAAAA (← ( )) mRNA A RNA mRNA A 120 mRNA ****** **** UAGCAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAGCAUAU GACUAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAA 30 A GCAUAUGACU 70 A VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.29/31
30. ( ) BNT162b2 mRNA RNA mRNA cap 5′ ( ) 2 3′ mRNA G C U Ψ VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.30/31
31. mRNA ( ) . . . VR 2021.9 & DAY2 – mRNA — 2021-09-19 – p.31/31