bins, not spots, at 3 different resolutions - 8μm bin size is recommended for analysis by 10x Genomics - Compare to 100μm distance between spots! - ~700k bins, compared to ~5k spots! - 2μm bin size is subcellular, and can be combined with segmentation to form cells “Bin level”: 8μm bins “Cell level”: Individual cells from combining 2μm bins 16μm bin border 8μm bin border 2μm bin border cell