Gut Microbiota signatures of fruit and vegetable intake

The project explained how the students analyze the 16rRNA data by using the Qiime2 pipeline & R program for the figures. I am honored to work and used dr. Frank Carbonero data the project title " Seeking meta-bolimic Associations related to the therapeutic diet (S.M.A.R.T) biomarker study.

Transcript

1. Gut Microbiota Signatures of Fruit and Vegetable Intake SUAD ALGARNI PhD Candidate Cell and Molecular Biology
2. Introduction • The important of Fruit & vegetable diet: o Disease prevention. oPart of a healthy diet. oIncreased F & V consumption reduced risk of: § Cardiovascular disease § Stroke § Cancers • Plant derived diatary fibre and/or flavonoids observed a protective role with gut microbiota
3. Objective: • To study the impact of fruit and vegetable (F&V) intake on gut microbiota. • Study design: oF&V intake: (low=one cup fruit & one cup of vegetable/day; high= 2 cups of fruit and 3 cups of vegetable/day) oNine days with a two-week washout period between treatments. oProvided stool samples. oBaseline(day 0) and three-day intervals (days 3,6, and 9) during the intervention.
4. Methods & Materials • Bacterial DNA extraction (stool sample) • PCR amplification and sequencing 16srRNA fast Q-file Operational Taxonomic Unit
5. Effect of High and Low Fruits and vegetables (F&V) diet on Bacterial Population 16s sequencing (phylum) 0.0 0.2 0.4 0.6 Actinobacteria Bacteroidetes Firmicutes Proteobacteria Verrucomicrobia Other variable value Day Day 0 Day 3 Day 6 Day 9 0.0 0.2 0.4 0.6 Actinobacteria Bacteroidetes Firmicutes Verrucomicrobia Other variable value Day Day 0 Day 3 Day 6 Day 9 High (F&V) group Low (F&V) group R (ggplot2)
6. Principal coordinate analysis (PCoA) plot of unweighted UniFrac distances between High &Low Fruits and vegetables (F&V) diet https://view.qiime2.org/ Day
7. Acknowledgement Caporaso’s group Dr.Greg Caporaso Dr.Nick Bokulich Matthew Dillon Evan Bolyen Dr. Frank Carbonero Dr.Daya Marasini