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Breeding methods in cross pollinated crops

Breeding methods in cross pollinated crops

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Deependra Dhakal

November 07, 2020
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  1. Breeding methods in Cross Pollinated crops Deependra Dhakal 2019/04/15 (updated:

    2020-10-08)
  2. Introduction

  3. Origin and distribution Mays is a diploid (2n = 20)

    and a monocot of the family Poaceae (Graminae) The genus has four species: Zea mays (cultivated corn and teosinte), Z. diploperennis Iltis et. al. (diploperennial teosinte), Z. luxurians, and Z. perennis (perennial teosinte). Zea mays spp. mays originated from teosinte (Z. mays spp mexicana/parviglumis) in western hemisphere about 7000-10000 years ago. Maize landraces are widely found across continents. Cultivated in more > 100 million ha in more than 125 developing countries. Maize was only introduced in africa 500 years ago. 3 / 32
  4. Landraces

  5. Landraces are defined mostly by their ear characteristic. Ear type

    is usually maintained by farmers through selection but crops biology itself plays a major role in structuring maize populations. American landraces: Tuxpeno (Native to Oaxaca chipas region): Tuxpeno Sequia (Early maturing, drought tolerant) Tuxpeno Crema (Resistant to foot rot disease, short, white kernel, strong stalk) Olotill (Native to Central Depression of Chipas; Shows good performance on unfertile soil; Has two mutant variants of gene Y1 (=psy1), phytoene synthase): Amarillo (Yellow) Blanco (White) Nal-tel (Native to Chiapas; Short growth cycle) Palomero Toluqeno (Adapted to high elevations and low temperature, resistant to Sitophus zeamais) Bolito (Native to Tehuacan region) Pepitilla, Bolita, Azul, Tlacoya (Excellent tortilla quality) 5 / 32
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  7. Indian landraces: Sikkim primitive (Murali): 5-9 ears on a single

    stalk (high prolificacy) Popcorn type Tassel long and drooping Perennial teosinte (Z. diploperennis) and Tripsacum dactyloides have relatively high resistance to striga weed. Striga resistant inbred: ZD05 Creolization: Process of encouraging gene exchange in maize landraces by growing different varieties in adjacent areas, and continually selecting seed of those varieties for replanting. 7 / 32
  8. Maize cytology and genomics Monoploids (haploids) may arise spontaneously by

    pathogenesis (unfertilized egg develops into a plant). Occasionally, paternal haploids develop by androgenesis. The average frequency of this event in corn is estimated at one per thousand kernels. These lines may be used to develop homozygous diploid inbred lines for hybrid production. They can also be used to convert inbred lines with male fertility to male sterile cytoplasm. Tetraploid corn was shown to have gigas effects. In 2012, genome of B73 (popular US Corn Belt inbred) and Palomero (Mexican popcorn landrace) was sequenced. In addition to the autosomes or normal or standard chromosomes (A-chromosomes), corn has supernumerary elements such as the B- chromosomes. Thousands of translocation events have been described in corn. They are used for locating genes on chromosomes. Maize gene for domestication (Tb1) mutant has 'Hopscotch' transposable element inserted into promoter region of the gene. More than 90% modern corn have this mutation. Mutation in Tga1 (Teosinte glume architecture1) is common. 8 / 32
  9. Xenia It is the immediate effect of pollen on the

    developing kernel. May be observed when two varieties differing in a single visible endosperm trait are crossed. Xenia occurs when the trait difference is conditioned by a dominant gene present in the pollen. However, when dominance is incomplete, xenia would occur when either vareity is the pollen parent. Endosperm characteristics distinguish some of the major corn groups. For example, starchy endosperm is dominant over sugary (sweet) and waxy. A cross of starchy x sugary exhibits xenia. Similarly, a cross of shrunken x non-shrunken endosperm, waxy x non- waxy endosperm, purple x colorless aleurone, and yellow x white (colorless) endosperm all exhibit xenia Whereas xenia may result from simple dominance gene action, the effect is different in some instances. In the cross of flinty x floury endosperm, the F1 is flinty (FFf). However, the reciprocal cross of floury x flinty endosperm produces an F1 with floury endosperm (ffF), indicating the ineffectiveness of the dominant allele (F) to overcome the double recessive (rr) floury genes. Similarly, xenia in aleurone color depends on the combined action of five dominant genes (designated A1, A2, C, R, and Pr). 9 / 32
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  11. Presence of male sterility genes (ms1, ms2, ..., ms20). Presence

    of transposable genetic elements 11 / 32
  12. Corn Classification Corn may be grouped into seven types on

    the basis of endosperm and glume characteristics as: Dent corn (Z. mays indentata) Flint corn (Z. mays indurate) Flour corn (Z. mays amylacea) Pop corn (Z. mays everta) Sweet corn (Z. mays saccharata) Pod corn (Z. mays tunicata) Waxy corn 12 / 32
  13. Heterotic grouping of maize germplasm Heterosis is related to the

    level of heterozygosity. Identification and development of heterotic groups of elite inbreds having different alleles at loci regulating productivity can contribute to hybrid performance. A heterotic group is germplasm that when crossed to germplasm from another heterotic group, tends to exhibit a higher degree of heterosis than when crossed to a member of its own group (Lee, 1995). Multiple heterotic patterns have been developed as a result of intensive elite line recycling and specific emphasis across breeding programs. Testcross performance with representative testers has been used to group large number of inbreds to known heterotic groups. Recently, DNA molecular markers have being effective for assigning inbreds to heterotic groups (Melchinger, 1999). 13 / 32
  14. Maize improvement (Response over cycles of selection)

  15. Hallauer (1999b) summarized the results of mass selection for earlier

    flowering in four tropical cultivars to reduce to photoperiod effects for possible use as germplasm sources for US Corn Belt breeding programs. Procedure: For each cycle of mass selection, 10,000–15,000 seeds were planted in an isolated field and the 250 earliest flowering plants were marked for selection. Selection was based on silk emergence with no selection for pollen shed. Response to selection was similar for each tropical cultivar (Table 1). Average linear response for earlier flowering was -3.3 days per cycle of selection. Correlated responses to selection for earlier flowering included reduced ear height and increased grain yield. Grain yields increased because of greater adaptation to temperate environments. Other correlated responses included reduced tassel size, reduced root and stalk lodging, reduced plant height, reduced infection by Ustilago maydis (DC.) Cda. Mass selection is a very cost effective method for adapting exotic sources to temperate environment, but the adapted exotic sources require greater breeding efforts within breeding programs. This is because mass selection does not include any intentional inbreeding to reduce the genetic load of deleterious recessive alleles and no testcrossing with adapted materials is involved to determine the combining ability of exotic materials with adapted materials. Note: Refer to @carena2009cereals, for details on overview of maize breeding 15 / 32
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  18. Narro (1990) evaluated Compuesto Selection Precoz after 15 cycles of

    half-sib recurrent selection for earliness. Compuesto Selection Precoz was formed by intermating 15 high yielding tropical germplasm. The goal of the selection program was to develop an early flowering, high yield cultivar for use in tropical areas. Selection was practiced at two locations in Mexico. After 15 cycles of selection for earliness, evaluations were conducted at 12 location (nine tropical and three temperate) to determine responses (direct and indirect) to selection for earlier flowering. Time from planting to flowering decreased 0.46 days per cycle (b = -10.46), which was less than that reported by Troyer and Brown (1972, 1976) and Hallauer (1999b). For the one temperate location (Ames, IA), direct response was -1.30 days per cycle, which aws similar to the data reported by Troyer and Brown (1972, 1976). Indirect response included reductions in grain yield, grain moisture, plant and ear heights and leaf area. Tropical cultivars grown in temperate environments are characterized as having tall stature, larger leaves, larger tassels, longer growing season because of photoperiodism, greater susceptibility to Ustilago maydis, lower grain yield, and consequently, a poor grain-to-stover ratio (<0.40). However, they have been successfully improved each cycle of selection when adapting to temperate environments. (See Table 1; reduced days to flowering of all tropical cultivars) 18 / 32
  19. Hainzelin (1998) used a combination of mass selection and backcrossing

    of exotic materials to adapted germplasm to reduce the effects of photoperiod. Photoperiod effects can also be reduced by crossing to a very early source followed by selection for adaptation (Gerrish, 1983; Holley and Goodman, 1988b) or by crossing improved unadapted sources followed by selection or by identifying photoperiod insensitive exotic sources (Oyervides-Garcia et al., 1985). Photoperiodism (it is believed to be controlled by few major genes and have been reduced by selection for earlier flowering) limits making direct comparisons between tropical and adapted cultivars in temperate environments. Although, selection still continues today to meet standards (grain yield, root and stalk strength, pest tolerance, maturity) to be useful for breeding programs in temperate US Corn Belt. Hence, crosses of tropical cultivars with earlier maturity materials, backcrosses to adapted recurrent parents, and testcrosses of tropical cultivars to adapted testers often have been used to determine the relative potential of tropical cultivars in temperate area breeding programs. The lesser the amount of tropical germplasm included in the evaluation trials, the greater the opportunity that useful germplasm from the tropical cultivars may be eliminated. This, of course, would detract from the original goals of introducing tropical materials to increase genetic diversity and introduce useful alleles from the tropical materials in temperate area breeding goals. 19 / 32
  20. The longest, continuous selection study in maize is the long-term

    selection experiment conducted at the University of Illinois in the open-pollinated cultivar Burr's White. The experiment was initiated in 1896 (Hopkins, 1899). Goal of the experiment was to determine if the chemical composition of the maize kernel could be altered by selection. Dudley and Lambert (2004) summarized 100 generations of selection for divergent protein and oil content; selection was effective in all instances. Dudley and Lambert (2004) presented a detailed history and analyses of the study that used a form of ear-to-row selection. 20 / 32
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  22. Half-sib family selection usually implicates the use of a tester

    to develop the half-sib families. Both of the original suggestions of recurrent selection used half-sib families. Jenkins (1940) used the source population as tester (GCA) whereas Hull (1945) suggested use of either an inbred or a single-cross as tester (SCA). Hence, the primary difference between the proposals of Jenkins (1940) and Hull's (1945) is the tester’s genetic base. Half-sib family selection for GCA was initiated in BSSS with IA13, a double-cross hybrid, as the tester, designating the population as BS13 (Hallauer, 1992). Half-sib family selection was continued in BS13 until 1970, when changed to S 1 –S 2 progeny selection. The half-sib family phase of recurrent selection in BS13 was effective in identifying inbred lines B14, B37, B73, and B84, which have been widely used in hybrids and as germplasm in pedigree breeding to develop recycled inbred lines (Mikel and Dudley, 2006). 22 / 32
  23. For intrapopulation improvement for both additive and dominance genetic effects,

    it seems full-sib family selection should have received greater interest. Moll and Hanson (1984), CIMMYT (Vasal et al., 1982), and NDSU (Carena, 2005a) have reported use of full-sib family selection. The CIMMYT maize breeding program has made greater use of full-sib family recurrent selection than others. One example is the selection for grain yield, days-to-silk, and plant height for eight tropical cultivars (CIMMYT, 1984). After four to five cycles of full- sib selection, average responses per cycle of selection were 12.7 q per ha (5.9%) for grain yield, -0.6 days-to-silking (-2.6%), and -1.0 cm (-4.6%) for reduced plant height. CIMMYT has also used full-sib family selection for reduced ear height, grain quality, and pest resistance that included within family selection with among family selection. 23 / 32
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  27. Hybrid testing and screening Hybrid testing in several environments representative

    of the target area is executed in several testing stages. A good example of testing stages within a commercial breeding program has been outlined by Smith et al. (1999): Stage 1. Testcross performance of experimental lines in few locations (e.g., five). Stage 2. Hybrid evaluation of selected lines in more hybrid combinations and locations (e.g., 20). Stage 3. Hybrid evaluation in about 50 locations on research plots in several hybrid combinations. Stage 4. Evaluation of best precommercial hybrids in about 75 research plot locations and about 200–500 on farm locations. Stage 5. Hybrid performance verification in about 75 research plot locations and 300–1500 on farm strip plot tests. 27 / 32
  28. Artificial pollination and hybridization Material requirement Preparation of female flower

    Pollination: Video on Pollination Method of Corn (University of Wisconsin-Madison) 28 / 32
  29. Common breeding objectives Grain yield Yield stability Agromorphological traits: Lodging

    resistance Resistance to ear drooping Husk covering Dry down Adaptation: Early maturity Drought resistance Cold tolerance Disease resistance Seed rot and seedling blight Root, stalk and ear rot Leaf blight or spots Smut Insect resistance Production quality 29 / 32
  30. Institutional initiatives in maize improvement Wellhausen Anderson Maize Genetic resources

    center of CIMMYT, El Baton, Mexico houses over 27,000 samples of maize seed which forms largest collection of landraces (24,191). Projects working on broadening the genetic base of cultivated maize: LAMP (Latin American Maize Program) USGEM (The US Germplasm Enhancement of Maize project) 30 / 32
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  32. References For a in-depth treatment of topic of diversity in

    maize germplasm, refer to [@prasanna2012diversity]. 32 / 32