Massive parallel processing of public high-throughput sequencing data and experiment of sharing data analysis environment

Transcript

1. େྔ/(4σʔλͷฒྻॲཧͱڞ༻εύίϯʹ͓͚Δ؀ڥߏஙͷࠓޙʹ͍ͭͯ ৘ใɾγεςϜݚڀػߏ ϥΠϑαΠΤϯε౷߹σʔλϕʔεηϯλʔ େా ୡ࿠ <t.ohta@dbcls.rois.ac.jp> ! prepared for Ҩ఻ݚDDBJεύίϯϢʔβձ

   July 22, 2014

2. Summary ‣ Ҩ఻ݚεύίϯΛར༻͠ެ։/(4σʔλશͯʹରͯ͠
 όονॲཧΛߦ͍ɼ%#ͷߏஙΛߦ͍ͬͯ·͢
   ! ‣ σʔλղੳύΠϓϥΠϯͷڞ༗ɾ࠶࣮ߦΛߦ͏ͨΊͷ
 7.ίϯςφΛར༻ͨ͠؀ڥߏஙͷௐࠪɾ։ൃΛߦ͍ͬͯ·͢

3. sra.dbcls.jp

4. ‣ ެ։/(4σʔλʹରͯ͠'BTU2$Λ࣮ߦ݁͠ՌΛճऩɾूܭ
   ‣ %-Մೳͳσʔλશ͕ͯର৅
   ‣ ʙ೥ొ࿥෼·Ͱ׬ྃ
   ‣ ૯σʔλ਺
   ‣ 

   
   4FRVFODF
   3VO
   TJOHMF
   PS
   QBJSFE
   ‣ ૯σʔλαΠζ
   ‣     
   5
   Ԙجର ެ։NGSσʔλͷϦʔυΫΦϦςΟDB

5. ‣ σʔλసૹ଎౓
   ‣ MGUQ
   NHFUʹΑΔ(#ͷσʔλసૹ
   
   
   
   Y
   ‣ ಉ࣌ฒྻ࣮ߦ਺
   ‣ $16
   
   $16
   

   Y طଘܭࢉػ؀ڥͱͷࠩ

6. ‣ ιϑτ΢ΣΞͷόʔδϣϯ؅ཧͷ໰୊
   ‣ ڞ༻؀ڥͰ͸Πϯετʔϧ͕೉͍͠৔߹΋͋Δ
   ‣ ݱঢ়͸౦େּݪ͞Μͷ-1.Λ࢖Θͤͯ௖͘ͳͲͰճආ
   ‣ IUUQXXXLBTBIBSBXTMQN
   ‣ େྔͷσʔλʹରͯ͠ͻͱͭͻͱͭख࡞ۀʁ

   ՝୊: ࿦จʹॻ͔ΕͨύΠϓϥΠϯΛ࠶ݱ͢Δ͜ͱ͕ࠔ೉

7. ‣ 7JSUVBM.BDIJOF 7. ΍ίϯςφͰ؀ڥ͝ͱղੳύΠϓϥΠϯΛڞ༗
   ‣ ΠϝʔδΛల։͙ͯ͢͠ʹղੳΛ࢝ΊΔ͜ͱ͕Ͱ͖Δ
   ‣ ؀ڥߏஙͱΠϝʔδڞ༗ͷٕज़ௐࠪ։ൃΛߦ͍ͬͯ·͢
   ‣ "NB[PO
   8FC
   4FSWJDFʹ͓͚Δ".*ͷڞ༗
   

   ‣ %PDLFS
   )VCʹ͓͚ΔίϯςφΠϝʔδͷڞ༗
   ‣ Ҩ఻ݚεύίϯͰ΋͜ΕΒͱޓ׵ੑΛ͍࣋ͨͤͨ σʔλղੳͷ࠶ݱੑΛ୲อ͢ΔͨΊͷղܾࡦ

8. ίʔυ΍ιϑτ΢ΣΞͱಉ͡Α͏ʹղੳ؀ڥΛެ։/ڞ༗

9. ίʔυ΍ιϑτ΢ΣΞͱಉ͡Α͏ʹղੳ؀ڥΛެ։/ڞ༗ $ docker run -d -p 8080:80 -t inutano/galaxy

10. ‣ Πϝʔδڞ༗Ͱ؀ڥ΁ͷґଘ͕ͳ͘ͳΔͱબ୒ࢶ͕૿͑Δ
    ‣ ࣗ෼Ͱߪೖͨ͠ܭࢉػ
    ‣ Ҩ఻ݚεύίϯͳͲͷڞ༻ܭࢉػϦιʔε
    ‣ "NB[PO
    8FC
    4FSWJDF "84 ͳͲͷ*OGSBTUSVDUVSF
    BT
    B
    4FSWJDF

    *BB4
    ‣ ܾΊख͸ಋೖͷίετͱϚγϯߏ੒ɼίετ
    ‣ "84ͷίετ͕͔ͳΓԼ͕ͬͨͨΊબ୒ࢶͱͯ͠ݱ࣮తʹ
    ‣ ϧʔνϯͳܭࢉ͸Ҩ఻ݚεύίϯͰ ͨͩͳͷͰ ܭࢉػϓϥοτϑΥʔϜͷબ୒

11. ॳظಋೖίετ ҡ࣋ίετ ߏ੒ͷॊೈੑ ৴པੑ/Ӭଓੑ ൿಗੑ ಛ௃ ݸผಋೖ ✕ ✕ ̋

    ˚ ̋ ࢿۚ͋Ε͹੍໿ͳ͠ ڞ༻ܭࢉػࢿݯ (NIGεύίϯ) ̋ ̋ ˚ ˚ ✕ DDBJͷDBͱ௚݁ IaaS (Ϋϥ΢υ) ̋ ˚ ̋ ˚ ˚ ඞཁͳ࣌ʹඞཁͳ͚ͩ ίετ΋೥ʑԼ͕Δ Ϣʔβࢹ఺Ͱͷ֤ܭࢉػ؀ڥͷϝϦοτൺֱ

12. Summary ‣ Ҩ఻ݚεύίϯΛར༻͠ެ։/(4σʔλશͯʹରͯ͠
 όονॲཧΛߦ͏͜ͱͰ%#ͷߏஙΛߦ͍ͬͯ·͢
    ! ‣ σʔλॲཧղੳύΠϓϥΠϯͷอଘӬଓԽ࠶࣮ߦΛߦ͏ͨΊͷ
 7.ίϯςφΛར༻ͨ͠؀ڥߏஙͱެ։%#ͷௐࠪɾ։ൃΛߦ͍ͬͯ·͢