Wear disposable gloves ▪ Tie tourniquet on patient’s arm ▪ Wipe the blood collection area with alcohol pad ▪ With the help of syringe, collect venous blood following aseptic precautions ▪ Then inoculate the sample immediately into bottle containing BHI, after disinfect the bottle
alcohol pad. ▪ Replace the needle with new one and insert the syringe in rubber cork. Don’t press the syringe as it leads to hemolysis. ▪ Mix the blood slowly in BHI broth bottle as not to allow the blood to clot in culture media because bacteria will get trapped in the clot. ▪ Incubate the blood culture bottle at 37°C for about 7 days and observe regularly for turbidity. ▪ If turbidity occurs, then perform sub- culture.
collect the first or last part of urine that comes out. This reduces the risk of the sample being contaminated with bacteria from: your hands, the skin around the urethra. ▪ This is the preferred type of specimen for culture and sensitivity testing because of the reduced incidence of cellular and microbial contamination.
collection. ▪ Refrigerated samples must be processed within 24 hours. ▪ Sample with borate preservative must be processed with in 24 hours. ▪ If the sample will not tested timely, it may leads to false positive results.