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Sample Collection For Culture Test

Sample Collection For Culture Test

How to collect blood and urine samples for culture test?

Maleeha Fatima

October 16, 2020
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  1. SAMPLE
    COLLECTION FOR
    CULTURE TEST
    By Maleeha Fatima

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  2. CULTURE TEST
    ▪ Medical laboratory test used to
    identify the presence of an
    microorganism in patient’s
    sample
    ▪ Culture- observable growth
    ▪ Media- substance required for
    the growth of culture

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  3. SAMPLE COLLECTION
    ▪ Main and important step
    ▪ Collected by well trained expert
    ▪ If does not collect properly,
    may leads to false positive
    result or damage to patient

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  4. BLOOD COLLECTION METHODS
    Arterial
    sampling
    Fingerstick
    sampling
    Venipuncture
    sampling

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  5. VENIPUNCTURE
    ▪ Collected from vein
    ▪ For laboratory testing i.e. culture
    test
    ▪ Performed by phlebotomist
    ▪ Easier than arterial sampling
    ▪ Drawn from the vein of top of the hand
    or inside of the elbow

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  6. REQUIREMENTS

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  7. METHOD
    ▪ Medical history of patient
    ▪ Find vein
    ▪ Wear disposable gloves
    ▪ Tie tourniquet on patient’s arm
    ▪ Wipe the blood collection area with alcohol
    pad
    ▪ With the help of syringe, collect venous
    blood following aseptic precautions
    ▪ Then inoculate the sample immediately into
    bottle containing BHI, after disinfect the
    bottle

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  8. Brain Heart Infusion (BHI)
    BHI broth is a highly nutritive
    media for cultivating wide variety
    of microorganisms.

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  9. ▪ Remove the cap and disinfect the rubber cork
    with alcohol pad.
    ▪ Replace the needle with new one and insert
    the syringe in rubber cork. Don’t press the
    syringe as it leads to hemolysis.
    ▪ Mix the blood slowly in BHI broth bottle as
    not to allow the blood to clot in culture
    media because bacteria will get trapped in
    the clot.
    ▪ Incubate the blood culture bottle at 37°C for
    about 7 days and observe regularly for
    turbidity.
    ▪ If turbidity occurs, then perform sub-
    culture.

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  10. NOTE
    ▪ Blood should be collect before
    antimicrobial treatment.
    ▪ The ratio of blood-to-nutrient broth
    must be correct.
    ▪ Check the sterility of BHI bottle before
    inoculation of blood.
    ▪ If the media is turbid, discard the
    bottle.

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  11. URINE SAMPLE COLLECTION
    ▪ Label a sterile, screw-top container.
    ▪ Wash your hands.
    ▪ Collect “mid-stream” urine in the
    container.
    ▪ Screw the lid of the container
    properly.
    ▪ Wash your hands thoroughly.

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  12. MID-STREAM URINE
    ▪ A mid-stream urine sample means you
    don’t collect the first or last part
    of urine that comes out. This reduces
    the risk of the sample being
    contaminated with bacteria from: your
    hands, the skin around the urethra.
    ▪ This is the preferred type of
    specimen for culture and sensitivity
    testing because of the reduced
    incidence of cellular and microbial
    contamination.

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  13. NOTE
    ▪ Processed the sample within 2 hours
    directly after collection.
    ▪ Refrigerated samples must be processed
    within 24 hours.
    ▪ Sample with borate preservative must be
    processed with in 24 hours.
    ▪ If the sample will not tested timely, it
    may leads to false positive results.

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  14. Thank you!

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