Sample Collection For Culture Test

How to collect blood and urine samples for culture test?

Transcript

1. SAMPLE
   COLLECTION FOR
   CULTURE TEST
   By Maleeha Fatima
   

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2. CULTURE TEST
   ▪ Medical laboratory test used to
   identify the presence of an
   microorganism in patient’s
   sample
   ▪ Culture- observable growth
   ▪ Media- substance required for
   the growth of culture
   

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3. SAMPLE COLLECTION
   ▪ Main and important step
   ▪ Collected by well trained expert
   ▪ If does not collect properly,
   may leads to false positive
   result or damage to patient
   

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4. BLOOD COLLECTION METHODS
   Arterial
   sampling
   Fingerstick
   sampling
   Venipuncture
   sampling
   

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5. VENIPUNCTURE
   ▪ Collected from vein
   ▪ For laboratory testing i.e. culture
   test
   ▪ Performed by phlebotomist
   ▪ Easier than arterial sampling
   ▪ Drawn from the vein of top of the hand
   or inside of the elbow
   

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6. REQUIREMENTS
   

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7. METHOD
   ▪ Medical history of patient
   ▪ Find vein
   ▪ Wear disposable gloves
   ▪ Tie tourniquet on patient’s arm
   ▪ Wipe the blood collection area with alcohol
   pad
   ▪ With the help of syringe, collect venous
   blood following aseptic precautions
   ▪ Then inoculate the sample immediately into
   bottle containing BHI, after disinfect the
   bottle
   

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8. Brain Heart Infusion (BHI)
   BHI broth is a highly nutritive
   media for cultivating wide variety
   of microorganisms.
   

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9. ▪ Remove the cap and disinfect the rubber cork
   with alcohol pad.
   ▪ Replace the needle with new one and insert
   the syringe in rubber cork. Don’t press the
   syringe as it leads to hemolysis.
   ▪ Mix the blood slowly in BHI broth bottle as
   not to allow the blood to clot in culture
   media because bacteria will get trapped in
   the clot.
   ▪ Incubate the blood culture bottle at 37°C for
   about 7 days and observe regularly for
   turbidity.
   ▪ If turbidity occurs, then perform sub-
   culture.
   

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10. NOTE
    ▪ Blood should be collect before
    antimicrobial treatment.
    ▪ The ratio of blood-to-nutrient broth
    must be correct.
    ▪ Check the sterility of BHI bottle before
    inoculation of blood.
    ▪ If the media is turbid, discard the
    bottle.
    

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11. URINE SAMPLE COLLECTION
    ▪ Label a sterile, screw-top container.
    ▪ Wash your hands.
    ▪ Collect “mid-stream” urine in the
    container.
    ▪ Screw the lid of the container
    properly.
    ▪ Wash your hands thoroughly.
    

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12. MID-STREAM URINE
    ▪ A mid-stream urine sample means you
    don’t collect the first or last part
    of urine that comes out. This reduces
    the risk of the sample being
    contaminated with bacteria from: your
    hands, the skin around the urethra.
    ▪ This is the preferred type of
    specimen for culture and sensitivity
    testing because of the reduced
    incidence of cellular and microbial
    contamination.
    

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13. NOTE
    ▪ Processed the sample within 2 hours
    directly after collection.
    ▪ Refrigerated samples must be processed
    within 24 hours.
    ▪ Sample with borate preservative must be
    processed with in 24 hours.
    ▪ If the sample will not tested timely, it
    may leads to false positive results.
    

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14. Thank you!
    

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