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Galaxy Community VM による NGSデータ解析

Tazro Inutano Ohta
June 05, 2015
Research
1
110

Galaxy Community VM による NGSデータ解析

第13回 がんとハイポキシア研究会 http://www.cancer-hypoxia.org/13th_conference.htm

Tazro Inutano Ohta

June 05, 2015
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Transcript

  1. Pitagora-Galaxy Project 2015 (BMBYZ$PNNVOJUZ7.ʹΑΔ /(4σʔλղੳ େాୡ࿠ ࢁதྒྷଠ ৘ใɾγεςϜݚڀػߏϥΠϑαΠΤϯε౷߹σʔλϕʔεηϯλʔ  ౦ژେֶઌ୺Պֶٕज़ݚڀηϯλʔ

    ͕ΜͱϋΠϙΩγΞݚڀձ

  2. Pitagora-Galaxy Project 2015 (BMBYZͱ͸ʁ (BMBYZ͸ɺແঈͰར༻Ͱ͖Δ౷߹σʔλղੳ؀ڥͰ͢ɻެ։͞Ε͍ͯΔιϑτ΢ΣΞ πʔϧ Λ૊Έ߹ΘͨղੳύΠϓϥΠϯͷߏஙɺ࣮ߦɺ࠶࣮ߦ΍݁Ռͷڞ༗͕؆୯ʹ ߦ͑ΔͨΊɺ/(4σʔλղੳʹ޿͘ར༻͞Ε͖࣮ͯͨ੷͕͋Γ·͢ 'JH 

    'JH (BMBYZͷΠϯλʔϑΣʔε 'JH (BMBYZ্ͰͷϫʔΫϑϩʔ

  3. Pitagora-Galaxy Project 2015 'JH XXXQJUBHPSBHBMBYZPSH 'JH ϐλΰϥɾΪϟϥΫγʔɾϓϩδΣΫτ (BMBYZ$PNNVOJUZ7. (BMBYZ$PNNVOJUZ+BQBO 1JUBHPSB(BMBYZ1SPKFDU

    ͸ɺ(BMBYZʹ͍ͭͯͷ৘ใڞ ༗ͷͨΊͷ΢ΣϒαΠτ XXXQJUBHPSBHBMBYZPSH Λ։ઃ͢Δͱڞʹɺ(BMBYZͱ ͦͷ্Ͱಈ࡞͢Δπʔϧ΍ϫʔΫϑϩʔΛઃఆࡁΈͷԾ૝؀ڥΛ഑෍͍ͯ͠·͢ɻҎ Լͷͭͷํ๏Ͱ͙͢ʹղੳϫʔΫϑϩʔΛ࣮ߦͰ͖·͢ɻ 'JH    ࣗ෼ͷ1$΍αʔόʔͰ࢖͑ΔԾ૝؀ڥ  ؆୯ͳղੳ͕Ͱ͖Δެ։ςετ༻αΠτ  Ϋϥ΢υͰىಈͰ͖ΔϚγϯΠϝʔδ

  4. Pitagora-Galaxy Project 2015 ಠࣗͷ֦ுػೳ 3FGFSFODF%PXOMPBE 'JH ͸ɺϦϑΝϨϯεσʔλ ྫ͑͹ɺ'"45"ήϊϜϑΝΠϧ΍ #8"ͷΠϯσοΫεϑΝΠϧ Λ؆୯ʹμ΢ϯϩʔυͰ͖ΔπʔϧͰ͢ɻ-PDBM'JMF

    #SPXTFS 'JH ͸ɺ(BMBYZ্ͰγʔέϯεσʔλϑΝΠϧΛ؅ཧͰ͖ΔπʔϧͰ͢ɻ֤ݚ ڀػؔͷطଘͷγʔέϯεσʔλΛݕࡧͯ͠(BMBYZʹΠϯϙʔτ͢Δ͜ͱ͕ग़དྷ·͢ɻ 'JH 3FGFSFODF%PXOMPBE 'JH -PDBM'JMF#SPXTFS

  5. Pitagora-Galaxy Project 2015 ৴པͰ͖ΔϫʔΫϑϩʔ (BMBYZ$PNNVOJUZ+BQBOʹ͸༷ʑͳେֶ΍ݚڀॴͷ(BMBYZϢʔβ͕ू·ΓɺͦΕͧ ΕͷݚڀࣨͰߏஙɾར༻͍ͯ͠ΔϫʔΫϑϩʔΛڞ༗͍ͯ͠·͢ɻͦΕͧΕͷϫʔΫ ϑϩʔʹ͍ͭͯͷৄࡉ͸XJLJ XJLJQJUBHPSBHBMBYZPSHXJLJ ʹ·ͱΊΒΕ͍ͯ·͢ɻ $PNNVOJUZ7.Λར༻͢Ε͹ɺ֤ݚڀࣨͰ࢖ΘΕ࣮ͨ੷ͷ͋ΔϫʔΫϑϩʔΛɺ͙͢

    ʹ࣮ߦ͢Δ͜ͱ͕Ͱ͖·͢ 'JH  ɻ 'JH ࢈૯ݚʹΑΔ#JTVMpUF4FRͷղੳϫʔΫϑϩʔ 'JH ཧݚʹΑΔ3/"4FRͷղੳϫʔΫϑϩʔ

  6. Pitagora-Galaxy Project 2015 কདྷల๬ ϐλΰϥΪϟϥΫγʔϓϩδΣΫτͰ ͸ɺ%PDLFSίϯςφΠϝʔδͷ࡞੒ ʹ΋औΓ૊ΜͰ͍·͢ɻ%PDLFSʹΑ Γɺ7.ΑΓ΋ߴ͍ύϑΥʔϚϯεͱɺ %PDLFSpMFʹΑΔ*OGSBTUSVDUVSFBT $PEFͷ࣮ݱʹΑΓɺσʔλղੳͷ࠶

    ݱੑΛ͞Βʹ޲্͢Δ͜ͱ͕Ͱ͖·͢ɻ কདྷతʹ͸ɺϢʔβχʔζʹ߹Θͤͨ ༷ʑͳܗͰͷԾ૝؀ڥͷ഑෍Λߦͬͯ ͍͖·͢ɻ