6 / group, N = 36 Discovery data Null: Alt: Models Cutoff Details • Rank DERs by area • 1000 permutations • Control FWER (≤ 5%) by max area per permutation Results 63,135 DERs 20.509 Corresponds to p-value 10-08 Jaffe et al, Nat. Neuroscience, 2015
group, N = 36 Replication data Null: Alt: Models Cutoff Details Per sample and per DER calculate average expression Results 50,650 DERs replicated Single F-statistic per DER p-value < 0.05 Jaffe et al, Nat. Neuroscience, 2015
change expression during brain development 1. derfinder permits discovery of novel expressed regions 2. we identified & validated gene expression changes in the developing brain 3. we have developed tools for reproducible/shareable reporting
LIBD Andrew Jaffe Jooheon Shin Nikolay Ivanov Amy Deep Ran Tao Yankai Jia Thomas Hyde Joel Kleinman Daniel Weinberger Harvard Rafael Irizarry Michael Love Funding NIH LIBD CONACyT México