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NGS Technologies: Illumina

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Avatar for Anton Nekrutenko Anton Nekrutenko
January 18, 2017
Education
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NGS Technologies: Illumina

Slides describing the principle behind Illumina technology

Avatar for Anton Nekrutenko

Anton Nekrutenko

January 18, 2017

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Transcript

  1. Illumina Illumina: Chemistry, Molecular Biology and Data (2018)

  2. Requirements ‣ Reversible termination ‣ Differentiation of nucleotides ‣ Ability

    to manipulate on solid support

  3. Preliminary studies

  4. Nucleotides Bentley et al. 2008

  5. Solid support Fedurco et al. 2006

  6. How it works

  7. The overall idea

  8. None
  9. None
  10. None
  11. None
  12. None
  13. None
  14. None
  15. None
  16. None
  17. None
  18. None

  19. Metzker et al. 2010

  20. Extending to paired ends

  21. None

  22. Multiplexing

  23. Detection system G,T A,C

  24. How it does not work

  25. Errors

  26. Phasing and Pre-phasing

  27. Illumina chastity filter

  28. Sequence Specific Errors Nakamura et al. 2011

  29. Schematic representation of the (a) inverted repeat and (b) enzyme

    preference for the SSE hypothetical mechanistic models. Nakamura K et al. Nucl. Acids Res. 2011;39:e90-e90 © The Author(s) 2011. Published by Oxford University Press.

  30. A typical run Minoche et al. 2011

  31. Coverage as function of GC% Minoche et al. 2011

  32. Low quality bases and alignment Minoche et al. 2011

  33. Context dependency Minoche et al. 2011

  34. None

  35. True seq PCR free