A Markov Random Fields Approach to the Gating of Flow Cytometry Data

Reasearch Students Conference Dublin 2016. Best talk prize awarded.

Transcript

1. A M A R KO V R A N D O M F I E L D S A P P ROA C H T O T H E GAT I N G O F F LO W C Y T O M E T R Y DATA K e v i n B r o s n a n , U n i v e r s i t y O f L i m e r i c k
2. P R E S E N TAT I O N OU T L I N E Flow Cytometry Existing methods Markov Random Fields Identifying Clusters Results Further Work
3. F LO W C Y T O M E T R Y …
4. W h at i s f lo w c y to m et r y ?
5. W h at i s f lo w c y to m et r y ? Flow
6. W h at i s f lo w c y to m et r y ? Flow Cyto
7. W h at i s f lo w c y to m et r y ? Flow Cyto metry
8. F lo w c y to m et r y d at a … Mean St. Dev. Min Max FSC.H 287.08 178.19 59 1, 023 SSC.H 251.83 186.65 11 1, 023 FL1.H 349.16 234.35 0 974 FL2.H 126.40 90.84 0 705 FL3.H 258.34 192.26 1 1, 023 FL1.A 73.46 195.15 0 1, 023 FL1.W 17.60 56.39 0 444
9. F lo w c y to m et r y d at a …
10. S p ars i t y … Statistic N Mean St. Dev. Min Max FSC.H 1, 545 71.40 44.53 14 255 SSC.H 1, 545 62.57 46.65 2 255 FL1.H 1, 545 86.93 58.58 0 243 FL2.H 1, 545 31.27 22.66 0 176 FL3.H 1, 545 64.20 48.03 0 255 FL1.A 1, 545 18.25 48.65 0 255 FL1.W 1, 545 4.33 14.01 0 111 time 1, 545 294.04 177.55 2 598
11. S p ars i t y …
12. E X I S T I N G M E T H O D S …
13. I n d u s t r y S t a n d ar d …
14. I n d u s t r y S t a n d ar d … 6= Expert 2 Expert 1
15. M o d e l B a s e d C lu s t er i ng …
16. t- d i s t r i b u t i o n M i x t u re s …
17. t- d i s t r i b u t i o n M i x t u re s …
18. M A R KO V R A N D O M F I E L D S …
19. X0,0 X1,0 X2,0 X3,0 X0,1 X1,1 X2,1 X3,1 X0,2 X1,2 X2,2 X3,2 X0,3 X1,3 X2,3 X3,3
20. X0,0 X1,0 X2,0 X3,0 X0,1 X1,1 X2,1 X3,1 X0,2 X1,2 X2,2 X3,2 X0,3 X1,3 X2,3 X3,3
21. X0,0 X1,0 X2,0 X3,0 X0,1 X1,1 X2,1 X3,1 X0,2 X1,2 X2,2 X3,2 X0,3 X1,3 X2,3 X3,3
22. X0,0 X1,0 X2,0 X3,0 X0,1 X1,1 X2,1 X3,1 X0,2 X1,2 X2,2 X3,2 X0,3 X1,3 X2,3 X3,3
23. Wo r k i ng E xam p le …
24. Wo r k i ng E xam p le …
25. I D E N T I F Y I N G C LU S T E RS …
26. C o n n e ct e d - C o m p o n e nt s …
27. C o n n e ct e d - C o m p o n e nt s …
28. R E S U LT S …
29. 6 -b i t co n f i g u r at i o n …
30. 6 -b i t co n f i g u r at i o n …
31. 6 -b i t co n f i g u r at i o n …
32. F U RT H E R WO R K …
37. T H A N K S F O R L I S T E N I N G ! A N Y Q U E S T I O N S ?