- Area of Interest: Data Integration & Visualization - University of California, San Diego Trey Ideker Lab - This presentation file is available on my Speaker Deck account - speakerdeck.com/keiono
on your machine. - Cytoscape 3.0.0 release is available, but at this point, it is for power users due to missing plugins. - Differences between 3.x and 2.x will be discussed
networks) 3.Analyze and Visualize Networks 4.Prepare for Publication - A specific example of this workflow: − Cline, et al. “Integration of biological networks and gene expression data using Cytoscape”, Nature Protocols, 2, 2366-2382 (2007). Cytoscape Workflow
bullet! - Understand Cytoscape Core Features - Research available Apps - Data pre-processing/post-processing may be required - Excel, R / Bioconductor, Scripts, Web Tools
workspace states into a Session File - Open means loading a Session file - You can open only one session at a time - Merge Session feature will be implemented in the future version of Cytoscape 3.x
Left-Click + Drag on Mac - Zoom - IN: Mouse Wheel UP - OUT: Mouse Wheel DOWN - Selection: Left-Click and Drag - Fit to Window - Selected region - Entire network
formats - Any table data can be imported to Cytoscape by Table Import function - Preparing your table data with widely-used ID is important for easy mapping
networks Maital Ashkenazi, Gary D. Bader, Allan Kuchinsky, Menachem Moshelion, David J. States Bioinformatics. 2008 June 15; 24(12): 1465–1466. Published online 2008 April 28. doi: 10.1093/bioinformatics/btn208 PMCID: PMC2427162
It is not necessary for filtering - Select → Nodes → Select All Nodes OR CTR-A - Sort by Drug Target (Click Column Name) - Select all rows with non-empty Drug Target cells - Right click and select from table
from table again - Now the selected nodes are druggable AND cancer genes. - Set values by Attribute Batch Editor. - Select Druggable genes again - Select rows with empty Group cells. These are druggable, non-cancer genes. - Group:* selects all
improve your visualization by following some simple rules - Info-Graphics != Data Visualization - Art : Science - Infographics 6:4 - Scientific Visualization 2:8
Saito, Michael E Smoot, Keiichiro Ono, Johannes Ruscheinski, Peng- Liang Wang, Samad Lotia, Alexander R Pico, Gary D Bader, Trey Ideker (2012) Nature Methods 9 (11) p. 1069-1076
Bend, Background Images - Server-side applications - Headless Mode (in beta status) - More advanced visualization by new rendering engines - For developers: Cleaner API
other networks are projections on that network. - Essentially a rooted tree - No way to duplicate nodes without sharing attributes - Cytoscape 3 - Allows multiple roots - Can have multiple trees - Each group of networks that shares a single root is called a collection
/ graduate students - Sponsored by Google - Students will be payed by Google - Please keep watching their web site - Application period for this year is mid April
real-world problems, National Resources for Network Biology (NRNB) is always open for collaboration! - NRNB Provides support for both of - Scientific Research - Application / Tool Development - nrnb.org
Protein–Protein Interactions. Part I. Experimental Techniques and Databases Shoemaker BA, Panchenko AR (2007) Deciphering Protein–Protein Interactions. Part I. Experimental Techniques and Databases. PLoS Comput Biol 3(3): e42.doi:10.1371/journal.pcbi.0030042 - Deciphering Protein–Protein Interactions. Part II. Computational Methods to Predict Protein and Domain Interaction Partners Shoemaker BA, Panchenko AR (2007) Deciphering Protein–Protein Interactions. Part II. Computational Methods to Predict Protein and Domain Interaction Partners. PLoS Comput Biol 3(4): e43. doi:10.1371/ journal.pcbi.0030043
A travel guide to Cytoscape plugins Rintaro Saito, Michael E Smoot, Keiichiro Ono, Johannes Ruscheinski, Peng-Liang Wang, Samad Lotia, Alexander R Pico, Gary D Bader, Trey Ideker (2012) Nature Methods 9 (11) p. 1069-1076 - Sample Protocol − Integration of biological networks and gene expression data using Cytoscape Cline, et al. Nature Protocols, 2, 2366-2382 (2007).