Somatostatin-Expressing Neurons Regulate Sleep Deprivation and Recovery: A Data-Driven Transcriptomic Analysis

This presentation outlines the findings from our paper published in Genes (2026). We investigated gene expression changes in the mouse cortex during Sleep Deprivation (SD) and Recovery Sleep (RS) using a fully data-driven approach.
Key Highlights:
• Methodology: Utilized Tensor Decomposition (TD)-based unsupervised feature extraction to identify gene expression patterns without prior hypotheses.
• Discovery: Identified distinct expression patterns where genes failed to normalize or "overshot" baseline levels during recovery sleep, contrary to traditional assumptions.
• Biological Insight: Enrichment analysis revealed that Somatostatin (Sst)-expressing neurons play a critical role in regulating sleep deprivation and recovery.
• Validation: Findings were validated using AlphaGenome, a generative AI, confirming that Sst enrichment is supported by genomic sequence predictions.
Reference: Kobayashi, K., & Taguchi, Y.-h. (2026). Somatostatin-Expressing Neurons Regulate Sleep Deprivation and Recovery. Genes, 17(1), 51. https://doi.org/10.3390/genes17010051

Transcript