of Genes With Altered Expression Caused by Coronavirus Infection and Screening of Candidate Drugs for SARS-CoV-2 Dear Y-h. Taguchi; We regret to inform you that your manuscript is inappropriate for bioRxiv. As an abundance of caution, bioRxiv is not currently posting predictions of drug/therapeutic efficacy/potential for treatment of COVID-19 that are based solely on in silico work, given concerns about drug availability and dangers to the general public. This manuscript should instead undergo rapid peer review at a journal before dissemination. This has been a difficult decision not arrived at lightly and we understand it may be disappointing, but we currently feel this is the most responsible course of action in these exceptional circumstances. Best regards, The bioRxiv team
l3k L1 L2 L3 HOSVD (Higher Order Singular Value Decomposition) テンソル分解に基づく教 N M K x ijk ≃∑ l 1 =1 L 1 ∑ l 2 =1 L 2 ∑ l 3 =1 L 3 G(l 1 l 2 l 3 )u l 1 i u l 2 j u l 3 k N: 遺伝子数の培養細胞の遺 (i) M: サンプル分解に基づく教数の培養細胞の遺 (j) K: 臓器数の培養細胞の遺 (k) xijk: 遺伝子 例
L 1 ∑ l 2 =1 L 2 ∑ l 3 =1 L 3 ∑ l 4 =1 L 4 G(l 1 l 2 l 3 l 4 )u l 1 j u l 2 k u l 3 m u l 4 i u l1j : l 1 種類の肺がんの培養目の培養細胞依存の肺がんの培養細培養細胞に依存性 u l2k : l 2 種類の肺がんの培養目の培養細胞依存の肺がんの培養細SARS-CoV-2感染させた。有無依存性 u l3m : l 3 種類の肺がんの培養目の培養細胞依存の肺がんの培養細biological replicate 依存性 u l4i : l 4 種類の肺がんの培養目の培養細胞依存の肺がんの培養細遺伝子依存性 G: 各項の重みの肺がんの培養細重みみ 目的:培養細胞の遺伝子発現プの提案種類ややbiological replicateに基づく教師なし 依らない(らない先進的(u l1j やu l3m は多すぎj,mに基づく教師なし依らない(らず一定値)一定できると同時に値ベクト)に対する新しい がガウス分布であ、得られた特異値SARS-CoV-2感染させた複数の培の提案有無で変わるわる新型コロナウイ (u l21 =-u l22 )に対する新しいに基づく教師なしなる新型コロナウイよう帰無仮説を使っなl 1 ,l 2 ,l 3 を感染させた。選びたいびたい
to exert cytotoxic and antiproliferative activities towards virus-transformed tumor cells, including myelocytomatosis virus and Rous sarcoma virus. Gentamicin Gentamicin is known to be a bactericidal antibiotic, it also exhibits antiviral activity Dibromochloromethane Dibromochloromethane was announced as a possible antiviral drug by the Agency forToxic Substances and Disease Registry
Since we do not know how many genes should be selected. We employed SAM and limma, which are gene selection specific algorithm (adjusted P-values are used ). t test SAM limma P>0.01 P≦0.01 P>0.01 P≦0.01 P>0.01 P≦0.01 Calu3 21754 43 21797 0 335 3789 NHBE 21797 0 21797 0 342 3906 A549 MOI 0.2 21797 0 21797 0 319 4391 MOI 2.0 21472 325 21797 0 208 4169 ACE2 expressed 21796 1 21797 0 182 4245 Statistical test Statistical test
P≦0.01 Calu3 7278 16432 NHBE 23383 327 A549 MOI 0.2 7858 15852 MOI 2.0 16279 7431 ACE2 expressed 16201 7509 After the publication of our paper, we have found the paper[*] that originally studied this GEO data was published (when we have done this study, only GEO data set was provides and no papers were published). The paper includes DESeq2 results. It is similar to limma; it detected most of genes as DEGs whereas it identified limited number of DEGs for NHBE cell lines [*]Daniel Blanco-Melo et al, Cell, 2020; 181(5): 1036-1045.e9. doi: 10.1016/j.cell.2020.04.026. Imbalanced Host Response to SARS- CoV-2 Drives Development of COVID-19 Statistical test Statistical test