Brockarchaeota, an archaeal phylum with unique methylotrophic pathways

FEMS-ASM Mäkelä-Cassell Award Awardee
8th Congress of European Microbiologist FEMS 2019

https://fems-microbiology.org/communities/valerie-de-anda-winner-of-the-fems-asm-makela-cassell-award-2019/

https://fems-microbiology.org/about_fems/network-and-activities/awards/fems-asm-makela-cassell-award/

Transcript

1. Valerie de Anda , Lin-Xing Chen, Nina Dombrowski, Zheng-Shuang Hua4, Hong-Chen Jiang, Jillian F. Banfield, Wen-Jun Li*, and Brett J. Baker* The University of Texas at Austin Marine Science Institute Port Aransas @val_deanda
2. Methylated compounds Methanol Methylamine Dimethylamine (DMA) Trimethylamine (TMA) CH3 NH2 CH3 NH2 CH3 CH3 CH3 OH C R Dimethylsulfide (DMS) CH3 S CH3 CH3 NH2 CH3 Important components of the global C, N, S Are derived from different sources such as phytoplankton, plants, and the decay of organic matter Abundant in the oceans, atmosphere and sediments
3. De Anda et al, Nature Microbiology, Submitted Methylated compounds in the context of carbon cycling Sources Sinks Radiotracer experiments
4. Integrating genome resolved metagenomics from extreme environments Hot springs Deep sea sediments De Anda et al, Nature Microbiology, Submitted Hua et al. , 2018 Nat Comm Dombrowski et al , 2018 Nat Comm 69.5° 63°C 56 °C 62 °C 10-33°C
5. 37 conserved single copy protein-coding gene tree TACK superphylum A new archaeal phylum that has been overlooked in metagenomic databases De Anda et al, Nature Microbiology, Submitted Emme L, et al., 2017 Nature T: AO (N cycle), mesophilic, low temperatures A: Thermophilic origin C: Thermo-acidophilic anaerobes K: Terrestrial and thermal environments
6. 37 conserved single copy protein-coding gene tree TACK superphylum A new archaeal phylum that has been overlooked in metagenomic databases De Anda et al, Nature Microbiology, Submitted 16S rRNA gene tree of sequences derived from metagenomic and rRNA-based diversity surveys
7. An overlooked archaea phylum globally distributed in anoxic environments De Anda et al, Nature Microbiology, Submitted Thomas Brock: Brockarchaeota
8. De Anda et al, Nature Microbiology, Submitted Methanol DMA TMA MtaA McrA MtaC CH3 S-CoM CH4 MtaA MtaB MtaC ATP Electron transfer MttB MttC MttA MtbA MtbB MtcC DMA TMA Methanol Brockarchaeota: methylotrophic metabolism » <Å̹ ̸ ȷ ̄ ă̊ ̄ īО ŏ ȷ Ř
9. Inferred energy conservation mechanisms in Brockarchaeota Methanol DMA TMA MtaA MtaB MtaC MtbB MtbC MtbA CoM-S-S-CoB CoM-SH+HS-CoB Fdox Fdred 2H2 4H+ HdrB HdrC MvhG MvhA HdrA MvhD 1-2,5,8 1-2,5 FrhG FrhA F420 H2 F420 H2 2H+ 1-4 1-4 MttB MttA MttC HS-CoM CH3 S-CoM Biosynthesis F420 CH4 H2 MtrA MtrH CH3 -S-CoM H2 H2 H2 +HS-CoM +HS-CoM +HS-CoM 3 1-3,7 1-4,6 1-4 1-4 2-4,7 ATP ADP ATPase 1-4,6-8 1-2,6 FrhB 1-2,6 1-5,7 1-2,5,7 1-2,5-7 4 NiFe H+ H2 FdOX Fdred 2e- 8 ? ? 2e- ? De Anda et al, Nature Microbiology, Submitted H2 -dependent methylotrophic pathway Electron donor Electron acceptors Energy conserved by a proton gradient generated by electron bifurcation PPi 2Pi H+ HppA 1-7 H+ H+ H+
10. Ethanol Methanol Acetaldehyde Acetate Acetyl-CoA Pyruvate Acetate ADP ATP YiaY AcdA Acs rTCA Glycolysis 1-5 1-5,8 1,3,5 Amino acids ProV 1-4 1-4,6-8 CAZyme Glucose MalK 7-8 Oligosacharides Polysaccharide Amino acids Inferred energy conservation mechanisms in Brockarchaeota De Anda et al, Nature Microbiology, Submitted ATP can be synthesized by substrate level phosphorylation via fermentation of methanol , ethanol or acetate Extracellular enzymes that can breakdown ohigh molecular-weight plant-derived polysaccharides: primarily xylanes (GH10, GH12), cellulose (GH5) and starch (GH57) PorA PorB PorG PorD
11. De Anda et al, Nature Microbiology, Submitted Brockarchaeota: a new key player in the global carbon cycle Sources Sinks Radiotracer experiments
12. De Anda et al, Nature Microbiology, Submitted Brockarchaeota: a new key player in the global carbon cycle Sources Sinks
13. Brett Baker The Baker microbial lab @archaeal Nina Dombrowski Jillian F. Banfield Lin-Xing Chen Thomas Brock Acknowledgements
14. Supplementary Information
15. Supplementary Information
16. Supplementary Information
17. Supplementary Information
18. Supplementary Information