x x x x x x x double stranded DNA SYBR green complex [fluorescing] concentration length of plug DNA is slowing down at moving front of SYBR green SYBR green is slowing down at moving front of DNA fluorescence length of plug
phosphonium brom ide p-nitrobenza ldehyde colourless NaOMe NO2 Me OH colourless P(Ph)3 + + Wittig reaction N + O O Cl Cl Cl Cl O O Cl Cl Cl N Enamine Chloranil blue 2,3,5-trichlor-6-(2-piperidin -1-yl)-[1,4]- benzoquinone Synthesis of a substituted aminovinyl-p-quinone SYNTHESIS
Gel electrophoretic photograph indicating that similar results were obtained with a 50ul microfabricated test device (mid-right three bands) as in much lager commercial instrument (mid-left two bands). The target sequence amplified was HIV.
a growing culture at various time intervals (by viable counts,dry weight of the biomass or optical density measurements) • Real-time monitoring • miniaturized systems • reduction of biological waste Conventional bulk growth techniques Microbiology microfluidic
0 1 2 3 4 5 0 10 20 30 40 50 60 70 80 90 100 sampling t (s) PMT sig. (V) 0hr 1hr 2hr a. Over the initial two hours, the data suggests that there is not a great deal of increase in the biomass. 0 1 2 3 4 5 0 10 20 30 40 50 60 70 80 90 100 sampling t (s) PMT sig. (V) 3hr 4hr b. After 3hr that there is any appreciable increase in the signal as indicated by figure b. At 4hrs, the signal has significantly increased.
UK European Commission, B Schlumberger, UK Casect, UK Agilent, D Forensic Lab, UK Asahi Kasei, Japan Lab of the Government Chemist, UK CSEM, Switzerland Amersham Pharmacia, UK Kodak, UK Glaxo Wellcome, UK Glaxo-Wellcome Heidelberg Instruments Hybaid MICROFABRICATION Alberta Microelectronics Centre, Canada Caliper Technologies, California MESA, University of Twente, The Netherlands CSEM, Switzerland !