on nanofluidics and why it is so great

Transcript

1. LATERNA MAGICA

2. REFE RENC E

3. LATERNA MAGICA

4. GLASS GLASS DEVICE

5. LATERNA MAGICA

6. THE OLDE BUNNY TRICK

7. MY LATEST AIR PUMP

8. THE OLDE BUNNY TRICK

9. THE OLDE BUNNY TRICK

10. microfluidics

11. Microf luidics

12. waves

13. None
14. None

15. On nanofluidics and why it’s so great S. Holmes, Baker

    Street, London

16. DNA research confinement & reading Tegenfeldt 2004, Craighead group Kasianowicz

    , 1996

17. Nanotechnology: spatial design instead of randomness Korda, 2002 Huang, 2004

    Continuous flow!

18. Thermal ratchets: employ noise Koss, 2003 Bader, 2004 Separation speed

    scales with 1/d2 !

19. Actin-myosin movement by ATP-biased Brownian motion (Kitamura 1999)

20. Rustom, 2004

21. Natura artis magistra (or: bottom-up and top-down) There’s plenty of

    sophistication at the bottom

22. Bottom-up: Aquaporins specific transport

23. proton transport blocked Water dipole reorientation  no dipole chain

     no Grotthus proton conduction Tajkhorshid et al., Science, 296 (2002) 525

24. Aquaporin http://www.ks.uiuc.edu/Research/aquaporins/

25. Bottom-up: Na+ K+ ATPase active transport through cell wall

26. Separation!

27. • Na+/K+ separating force (Energy / distance ) : 1ATP

    / (membrane thickness) = 14 kT / 4 nm  14 pN • Pumps against E-field of 70 mV / 4 nm = 1.75e7 V/m • Electrophoresis: max. 2e5 V/m  0.03 pN on unit charge • Almost isothermal, ~ 100% efficiency • Energy dissipation only where needed
28. None

29. Combining top/down and bottom/up Cornell, 1997

30. Why nanofluidics is so great • Single molecule studies •

    Freedom of nanoarchitecture instead of random separation structure – Sophisticated sieving (continuous flow), ratchets • From nature: active transport for separation – efficient – high separating field gradients – specific

31. Now, isn’t that great???

32. None