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Roscoff Culture Collection - User Committee 2020

Cdea0b090865961754c9ce266aa37c27?s=47 Daniel Vaulot
December 06, 2020

Roscoff Culture Collection - User Committee 2020

Cdea0b090865961754c9ce266aa37c27?s=128

Daniel Vaulot

December 06, 2020
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  1. Roscoff Culture Collection Collection de Cultures de Roscoff Station Biologique

    de Roscoff FR2424 et UMR7144 Director : Ian Probert, Curator: Priscillia Gourvil Scientific director: D. Vaulot
  2. The Roscoff Culture Collection • Set up in 1998 from

    research collection • 5,597 strains (Dec 2020) • Large phylogenetic coverage • Large coverage of oceanic regions (arctic to tropics)
  3. A few numbers for 2020 • 34,190 transfers (660 strains

    per week) • 565 L of medium • 16 visitors to RCC facility • 191 orders (796 strains) 21% decrease compared to 2019 (243 orders and 1099 strains) • Strains sent to 20 countries The Roscoff Culture Collection
  4. Personnel RCC Ian PROBERT IR SU Director Priscillia GOURVIL IE

    CNRS Curator Martin GACHENOT AI CDD SU Assistant Curator Léna GOUHIER AI CDD SU Assistant Curator Valeria JIMENEZ CR CDD SU Projet Moore Support Daniel VAULOT DR CNRS Scientific Director Florence LE GALL IE CNRS Isolation Fabienne JALABERT IR CNRS Microscopy Dominique MARIE IR CNRS Flow cytometry sorting Christian JEANTHON DR CNRS Bacteria Anne-Claire BAUDOUX CR CNRS Viruses
  5. • Culture space – 3 culture rooms (20°C/13°C/4°C) – 14

    culture cabinets – 1 transfer laboratory – 1 cryopreservation lab • Cryopreservation equipment – KryoPlaner Progressive freezer – 2 -150°C freezers – Liquid nitrogen tanks • 4 microscopes equipped for digital imaging • 5 flow cytometers – FACSAria – high speed sorter (2003) – FACSCanto – analysis (2008) – Beckmann Coulter Quanta – analysis (2009) – Accuri C6 – analysis (2011) – Guava - analysis (2016) Infrastructure
  6. 5,597 strains Overview of RCC

  7. Strains added per year Green Edge MACUMBA Ian

  8. New strains 2020 Deposits Bergen

  9. New strains 2020

  10. RCC sequences in Genbank

  11. Papers 2020 69 papers published in 2020 using RCC strains

    (55 in 2019, 54 in 2018, 57 in 2017)
  12. Distribution 5,305 strains available 12 098 cultures distributed

  13. Distribution ca. 35k € in 2020

  14. Cryoconservation Number of cryopreserved strains : 1536 190 strains cryopreserved

    in 2020 Mostly diatoms but also parasites and bacteria Test new protocols for cryo-recalcitrant strains Cryptophyceae, Dinoflagellate 3 Cryoprotectants, 2 concentrations No positive results Cryopreservation service 2 orders : Cyanobacteria, Plantae (Helichrysum stoechas) Private company EBB Project: Intercalibration event: 7 laboratories, 2 strains (I.galbana and P.tricornutum) Publication Paredes E., Ward A., Probert I., Gouhier L., Campbell C.N. (2021) Cryopreservation of Algae. In: Wolkers W., Oldenhof H. (eds) Cryopreservation and Freeze-Drying Protocols. Methods in Molecular Biology, vol 2180. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0783-1_32
  15. Other services Culture Media 3 media (f/2,K,PCRS11-RS) -> 27 litres

    ordered (15L in 2019) Algal Strain Identification 4 orders (private companies) - 18 strains Freshwater samples, powder
  16. User feedback User survey Link sent with order and then

    sent again when billing Answer rate: 13% (9,4% in 2019) Customers are globally satisfied Knowledge through publications Feedback via website - Customers interrested in phytoplanckton course (cultivation and taxonomy) - Remarks about low culture concentration
  17. Quality Management System Under ISO 9001: 2015 regulation Framework of

    EMBRC-France Certification postponed to 2021 EBB Workshop (virtual) 1,5 days /4 laboratories: Introducing a common quality management system - Define a set minimum set of QM requirements for culture collections and biobanks belonging to EBB. - Need and advantages of advancing QMS - Agree on a number or steps for culture collections to implement that set of requirements 7 anomalies Customs retention Lighting system
  18. Novelties 2020 • Basic 3 day culturing/identification course (postponed to

    March 2021) • Website statistics tools • Logo
  19. 1 5 3 4 2 8 6 7 Actuel

  20. RCC Outlook 2021 • Projects: ANR Phenomap - 418K€ FR2424,

    ECOMAP-GOSEE, Ifremer Concarneau (LERBO) - Phenotypic description : strains, single-cell, FISH - RCC postdoc (and PhD?) - Sampling missions: Villefranche, Singapore?, +? - On-line identification guide SMAL: Scent of Microalgae - 22 K€ Ifremer Brest and Nantes Microalgae volatilome characterisation to identify aromas of industrial interest for the fragrance market • Basic culturing/identification course (March 2021) • Advanced Phytoplankton Course 13 (Naples 2021 ??) • Purchase of a new progressive freezer • Development of educational kits / photobioreactor services ? • Algal monoclonal antibodies ? • Internal tarif for collaborations / education ?