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Roscoff Culture Collection - User Committee 2020

Daniel Vaulot
December 06, 2020

Roscoff Culture Collection - User Committee 2020

Daniel Vaulot

December 06, 2020
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  1. Roscoff Culture Collection
    Collection de Cultures de Roscoff
    Station Biologique de Roscoff
    FR2424 et UMR7144
    Director : Ian Probert, Curator: Priscillia Gourvil
    Scientific director: D. Vaulot

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  2. The Roscoff Culture Collection
    • Set up in 1998 from research collection
    • 5,597 strains (Dec 2020)
    • Large phylogenetic coverage
    • Large coverage of oceanic regions (arctic to tropics)

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  3. A few numbers for 2020
    • 34,190 transfers (660 strains per week)
    • 565 L of medium
    • 16 visitors to RCC facility
    • 191 orders (796 strains)
    21% decrease compared to 2019 (243 orders and 1099 strains)
    • Strains sent to 20 countries
    The Roscoff Culture Collection

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  4. Personnel
    RCC
    Ian PROBERT IR SU Director
    Priscillia GOURVIL IE CNRS Curator
    Martin GACHENOT AI CDD SU Assistant Curator
    Léna GOUHIER AI CDD SU Assistant Curator
    Valeria JIMENEZ CR CDD SU Projet Moore
    Support
    Daniel VAULOT DR CNRS Scientific Director
    Florence LE GALL IE CNRS Isolation
    Fabienne JALABERT IR CNRS Microscopy
    Dominique MARIE IR CNRS Flow cytometry sorting
    Christian JEANTHON DR CNRS Bacteria
    Anne-Claire BAUDOUX CR CNRS Viruses

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  5. • Culture space
    – 3 culture rooms (20°C/13°C/4°C)
    – 14 culture cabinets
    – 1 transfer laboratory
    – 1 cryopreservation lab
    • Cryopreservation equipment
    – KryoPlaner Progressive freezer
    – 2 -150°C freezers
    – Liquid nitrogen tanks
    • 4 microscopes equipped for digital imaging
    • 5 flow cytometers
    – FACSAria – high speed sorter (2003)
    – FACSCanto – analysis (2008)
    – Beckmann Coulter Quanta – analysis (2009)
    – Accuri C6 – analysis (2011)
    – Guava - analysis (2016)
    Infrastructure

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  6. 5,597 strains
    Overview of RCC

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  7. Strains added per year
    Green Edge
    MACUMBA Ian

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  8. New strains 2020
    Deposits
    Bergen

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  9. New strains 2020

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  10. RCC sequences in Genbank

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  11. Papers 2020 69 papers published in 2020 using RCC strains
    (55 in 2019, 54 in 2018, 57 in 2017)

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  12. Distribution
    5,305 strains available
    12 098 cultures distributed

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  13. Distribution
    ca. 35k € in 2020

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  14. Cryoconservation
    Number of cryopreserved strains : 1536
    190 strains cryopreserved in 2020
    Mostly diatoms but also parasites and bacteria
    Test new protocols for cryo-recalcitrant strains
    Cryptophyceae, Dinoflagellate
    3 Cryoprotectants, 2 concentrations
    No positive results
    Cryopreservation service
    2 orders : Cyanobacteria, Plantae (Helichrysum stoechas)
    Private company
    EBB Project:
    Intercalibration event:
    7 laboratories, 2 strains (I.galbana and P.tricornutum)
    Publication
    Paredes E., Ward A., Probert I., Gouhier L., Campbell C.N. (2021) Cryopreservation of Algae. In: Wolkers W., Oldenhof
    H. (eds) Cryopreservation and Freeze-Drying Protocols. Methods in Molecular Biology, vol 2180. Humana, New York,
    NY. https://doi.org/10.1007/978-1-0716-0783-1_32

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  15. Other services
    Culture Media
    3 media (f/2,K,PCRS11-RS)
    -> 27 litres ordered (15L in 2019)
    Algal Strain Identification
    4 orders (private companies) - 18 strains
    Freshwater samples, powder

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  16. User feedback
    User survey
    Link sent with order and then sent again when billing
    Answer rate: 13% (9,4% in 2019)
    Customers are globally satisfied
    Knowledge through publications
    Feedback via website
    - Customers interrested in phytoplanckton course (cultivation and
    taxonomy)
    - Remarks about low culture concentration

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  17. Quality Management System
    Under ISO 9001: 2015 regulation
    Framework of EMBRC-France
    Certification postponed to 2021
    EBB Workshop (virtual)
    1,5 days /4 laboratories: Introducing a common quality management system
    - Define a set minimum set of QM requirements for culture
    collections and biobanks belonging to EBB.
    - Need and advantages of advancing QMS
    - Agree on a number or steps for culture collections to implement
    that set of requirements
    7 anomalies
    Customs retention
    Lighting system

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  18. Novelties 2020
    • Basic 3 day culturing/identification course (postponed to March 2021)
    • Website statistics tools
    • Logo

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  19. 1
    5
    3
    4
    2
    8
    6
    7
    Actuel

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  20. RCC Outlook 2021
    • Projects:
    ANR Phenomap - 418K€
    FR2424, ECOMAP-GOSEE, Ifremer Concarneau (LERBO)
    - Phenotypic description : strains, single-cell, FISH
    - RCC postdoc (and PhD?)
    - Sampling missions: Villefranche, Singapore?, +?
    - On-line identification guide
    SMAL: Scent of Microalgae - 22 K€
    Ifremer Brest and Nantes
    Microalgae volatilome characterisation to identify aromas of industrial interest for
    the fragrance market
    • Basic culturing/identification course (March 2021)
    • Advanced Phytoplankton Course 13 (Naples 2021 ??)
    • Purchase of a new progressive freezer
    • Development of educational kits / photobioreactor services ?
    • Algal monoclonal antibodies ?
    • Internal tarif for collaborations / education ?

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