SMRT Sequencing Overview

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1. FIND MEANING IN COMPLEXITY © Copyright 2013 by Pacific Biosciences of California, Inc. All rights reserved. SMRT® Sequencing Overview
2. The PacBio® Difference Observes single molecules in real time to provide high-throughput SMRT® Sequencing of DNA and base modifications simultaneously • Generate finished genomes • Discover a broad spectrum of base modifications • Characterize complex variations • Extraordinarily long read lengths • Extremely high accuracy • Exquisite sensitivity • Shortest run time • Least GC bias • No amplification bias
3. Single Molecule, Real-Time (SMRT®) DNA Sequencing PacBio® RS II Trace SMRT® Cells Zero-Mode Waveguides Phospholinked Nucleotides
4. SMRT® Technology Overview 4
5. Signal Processing and Base Calling Converting pulses of light into DNA bases and kinetic measures 5
6. Typical PacBio® RS II Results
7. SMRT® Sequencing Accuracy Data generated with P4-C2 chemistry on PacBio® RS II; Analyzed using Quiver with 2.0.1 SMRT® Analysis Perspective: Understanding SMRT Sequencing Accuracy
8. Integrated End-to-End Solutions Easy, user-friendly, web-based solutions Streamlined data analysis and viewing Support for novice and expert users
9. Products and Workflow 9 DNA Template Kit DNA Polymerase Binding Kit MagBead Kit Library Preparation No amplification required PacBio® RS II RS Remote RS Touch SMRT Cells DNA Sequencing Kit Instrument Run Sequencing time 30 to 120 min per SMRT® Cell SMRT Analysis SMRT Portal SMRT View Data Analysis Open source, open standards
10. Targeted Sequencing: High-Resolution Insights Exquisite sensitivity and specificity to fully characterize genetic complexity – Multi-kilobase reads – 99.999% consensus accuracy – Linear variant detection to <0.1% frequency – Access to the entire genome SNP Detection and Validation Repeat Expansions Compound Mutations and Haplotype Phasing Minor Variants Detection www.pacb.com/target Full-Length Transcripts and Splice Variants
11. De Novo Assembly: Reduce Ambiguities Complete microbial genomes and improve assemblies of larger organisms • 99.999% accuracy • Highest N50 • Fewest fragments • Uniform coverage • Span highly repetitive regions • Detect structural variation • Improve gene models Read lengths up to 20 kb, unbiased genome coverage, and high accuracy Finished bacterial genome www.pacb.com/denovo
12. Base Modification: Discover the Epigenome Detect base modifications using the kinetics of the polymerization reaction during normal sequencing ® www.pacb.com/basemod
13. Customer Evidence: More Than 45 Customer Publications Read Customer Publications